Construction of Nucleic Acid antibodies to PrP(sc)

描述(申请人摘要)：我们建议开发一种方法 产生针对感染性疾病的可扩增核酸(NA)抗体 与PrP(Sc)有关的粒子。NA抗体有能力 结合具有高亲和力和特异性的蛋白质。可扩增的NA抗体 将通过使用SELEX过程的变体生成 Q-β复制酶活性的优势取代了三个步骤 SELEX(逆转录、聚合酶链式反应扩增和RNA转录)。 此外，编码蛋白质特异性结合的所选序列 元件包含在从Q-β复制酶衍生的RNA载体中 模板(MNV)，提供了指数扩增的手段。这是一项新的 方法极大地简化和改进了selex过程，使其 在普通分子生物学实验室中的应用。PrP的累积(Sc) 导致无法治愈的海绵状脑炎，通常是获得性脑炎 通过食用受污染的牛肉。由于目前对普恩病毒的检测需要 几年来，由于美国的病例数量正在上升，有 迫切需要一种廉价、快速的诊断方法。NA抗体 可被q-beta复制酶扩增的PrP(Sc)的特异性对于 使用该公司的核心开发一种高度敏感的诊断分析方法， 专有技术。 建议的商业应用： 我们的选拔过程可能会导致NA抗体生产服务的发展 用于研究或为酶Q-beta创建市场而出售的试剂盒 复制酶。可扩增的PrP(Sc)NA抗体可作为治疗 开发一种廉价的诊断分析方法。

DESCRIPTION (Applicant's abstract): We propose to develop a method for generating amplifiable nucleic acid (NA) antibodies specific for the infectious particle responsible for prion disease, PrP(sc). NA antibodies have the ability to bind proteins with high affinity and specificity. Amplifiable NA antibodies will be generated by using a variation of the SELEX procedure that takes advantage of the activity of Q-beta replicase which replaces three steps in SELEX (reverse transcription, PCR amplification and RNA transcription). Moreover, the selected sequence that encodes the protein specific binding element is contained within an RNA vector derived from a Q-beta replicase template (MNV), providing the means for exponential amplification. This new method greatly simplifies and improves upon the SELEX procedure, enabling its application in the common molecular biology laboratory. Accumulation of PrP(sc) in the brain leads to untreatable spongiform encephalitis, typically acquired through consumption of contaminated beef. Since current tests for prions takes years, and since the number of cases in the United States is on the rise, there is an urgent need for an inexpensive, rapid diagnostic assay. NA antibodies specific for PrP(sc) that are amplifiable by Q-beta replicase are ideal for the development of a highly sensitive diagnostic assay using the Company's core, proprietary technology. PROPOSED COMMERCIAL APPLICATION: Our selection process may lead to a NA antibody production service, the development of kit for sale for research purposes or the creation of a market for the enzyme, Q-beta replicase. Amplifiable NA antibodies for PrP(sc) can be used as the raw material to develop an inexpensive diagnostic assay.