Automated Monitoring of Chemotactic Cell Movement

描述(申请人提供)：ECIS/出租车技术可以检测到 通过琼脂糖基转移到小电极上的细胞 它们传递给电路的电阻，因为它们覆盖了 小电极。该系统适用于小区吞吐量的快速分析 在趋化因子和其他刺激细胞的因素存在时的行为 有动静。这项技术作为替代技术具有重要的优势 传统的细胞运动测量方法。首先，它允许对细胞进行评估 在没有预先确定特定终点的情况下，对刺激的反应。 第二，ECIS/出租车环境更贴近自然生物 环境。第三，可以根据数据对数据进行量化评估 电路电阻的变化。我们在第一阶段的结果是 项目清楚地证明了这项技术可以用来测量 细胞对趋化因子的反应及药物对其的影响 趋化反应。在这项计划的第二阶段，我们会发展 可用于评估趋化和趋化动力学的方案 哺乳动物白细胞的反应和原型的评价方法 趋化作用的拮抗剂。 建议的商业应用： ECIS/出租车技术将立即在寻找抗趋化药物方面发挥作用 抗炎药、趋化药(新的趋化因子)、激动剂 已知的趋化因子(促炎因子)和运动刺激物(促炎因子 代理)。

DESCRIPTION ( provided by applicant): The ECIS/taxis technology can detect cells that move to a small electrode through an agarose matrix by the resistance that they impart to an electrical circuit as they cover parts of the small electrode. This system is amenable to rapid throughput analysis of cell behavior in the presence of chemokines and other agents that motivate cell movement. This technology has important advantages as an alternative to traditional measures of cell movement. First, it enables the evaluation of cell responses to stimuli without the preestablishment of a specific endpoint. Second, the ECIS/taxis environment more closely reflects the natural biological environment. Third, the data can be quantitatively assessed according to changes in the resistance of the circuit. Our result in the Phase I of this project clearly demonstrate that this technology can be used to measure the response of cells to chemotactic factors and effects of drugs on the chemotactic response. In Phase II of this project, we will develop the protocols that can be used to evaluate the chemotactic and chemokinetic responses of mammalian leukocytes, and the methods of evaluation of prototype antagonists of chemokinesis. PROPOSED COMMERCIAL APPLICATION: The ECIS/taxis technology will be immediately useful in the search for anti-chemotactic agent (anti-inflammatory agents), chemotactic agents (new chemokines), agonists of known chemokines (pro-inflammatory agents), and stimulators of motility (pro-inflammatory agents).