Light regulation of gene expression and inter-tissue signalling

基因调控是许多生物过程的核心。本项目旨在研究光信号如何在组织水平上调节基因，以确定植物的整个生物体反应。利用卡森实验室开发的模式生物拟南芥和特定转基因品系，该项目将使用下一代测序和生物信息学分析来询问光依赖基因在组织特异性水平上的调控。该项目将检查现有的基因表达数据，并生成新的数据集用于分析，目的是确定组织特异性调节网络。分子技术的结合，包括定量RT-PCR、报告基因测定、Western blotting和突变分析，将检查这些网络的组成部分在调节光反应中的作用。

Gene regulation is at the core of many biological processes. This project aims to examine how light signals regulate genes at the tissue level to determine whole organism responses in plants. Using the model organism Arabidopsis thaliana and specific transgenic lines developed in the Casson laboratory, this project will use next generation sequencing and bioinformatics analysis to interrogate light-dependent gene regulation at the tissue specific level. The project will examine both existing gene expression data and generate novel data sets for analysis with the aim of identifying tissue specific regulatory networks. A combination of molecular techniques including quantitative RT-PCR, reporter gene assays, Western blotting and mutant analysis will then examine the role of components of these networks in regulating light responses.