DNA repair and mutagenesis at replication forks: analysis of HelQ and Rrm3 helicases

该项目的重点是人类DNA修复酶Helq。HELQ功能障碍与某些癌症的发生有关，其机制尚不清楚，但似乎与HELQ与RAD51和RAD51辅酶相互作用的能力有关。轮换将花费几周时间通过(A)定点突变对ATPase、DNA结合和解旋酶活性的影响和/或(B)解旋酶蛋白质与RAD51及其类似物的相互作用来进行解旋酶的生化分析。

The project focuses on HelQ, a human DNA repair enzyme. Malfunctions in HelQ are associated with the onset of some cancers, by mechanisms that are unclear but seem to relate to the ability of HelQ to interact with Rad51 and Rad51 paralogue enzymes. The rotation will spend a few weeks doing biochemical analysis of helicase enzymes by (a) the effects of site-directed mutagenesis on ATPase, DNA binding and helicase activity and/or (b) analysis of helicase protein-protein interactions with Rad51 and its paralogues.