Specific Oligomers for Rapid Detection of Animal Pathogens via PCR and RT-PCR

利用聚合酶链式反应(PCR)建立了一组鉴定动物重要的细菌、病毒和真菌病原体的分子方法。这一能力已被证明是传统微生物技术的宝贵补充，特别是在检测难以培养的微生物或埋藏在档案材料中的微生物方面。这些程序的发展也提供了一种将病原体载量与动物的年龄和病程相关联的方法。其中许多技术的效果仍在评估中。

Molecular methods employing the polymerase chain reaction (PCR) were developed to identify a panel of 30 important bacterial, viral and fungal pathogens of animals. This capability has proven to be a valuable adjunct to traditional microbiological techniques especially for detecting microbes difficult to culture or those buried in archival material. Development of these procedures has also provided a means for correlating pathogen load with the animal's age and the course of disease. The efficacy of many of these techniques is still being evaluated.