MICROSATELLITE ANALYSIS OF RODENTS IN THE NIH REPOSITORY

该项目的目标是发现微卫星制图是否提供了一种足够强大的遗传谱验证方法，以取代传统的同酶分析进行常规监测。因此，我们目前正在使用5个微卫星引物集来扩增我们从54个不同啮齿类动物菌株的尾巴上制备的DNA，这些菌株被安置在NIH储存库中或冷冻保存。到目前为止，我们已经发现，PCR方法是一样的，如果不是更多，敏感和更省力的比更传统的生化技术。然而，尽管微卫星方法的劳动强度低于DNA指纹，但至少目前来看，后者似乎比前者具有更高的灵敏度。

The goal of this project is to discover if microsatellite mapping provides a method for genetic profile verification robust enough to supplant conventional allozyme analysis for routine monitoring. Consequently, we are currently using five microsatellite primer sets to amplify DNA we have prepared from tails of 54 different rodent strains housed or cryopreserved in the NIH repository. To date, we have found the PCR approach to be as, if not more, sensitive and less laborious than the more conventional biochemical technique. However, although the microsatellite method is less labor intensive than DNA fingerprinting, the latter appears, at least for now, to have greater sensitivity than the former.