DNA Fingerprinting Rodents in the NIH Repository

NIH动物遗传资源（NIHAGR）中保存的啮齿动物品系的DNA指纹图谱和组织库已经变得更加紧迫，因为大多数品系都处于冷冻保存的过程中。为了监测NIHAGR近亲繁殖计划的成功，并为利用这些菌株的研究人员保持一致的标准，我们的实验室将代表性后代的组织加工成超纯DNA。 然后将该材料用于多位点探针的DNA指纹分析，以确认使用同工酶分析的常规遗传监测结果。除了大鼠和小鼠的数据外，我们还证明了可以通过该技术区分从封闭繁殖群繁殖的仓鼠、豚鼠和猪。

The DNA fingerprinting and banking of tissues of the rodent strains maintained in the NIH Animal Genetic Resource (NIHAGR) has taken on a greater urgency since most of the strains are in the process of being cryopreserved. To monitor the success of inbreeding programs at the NIHAGR as well as to maintain consistent standards for investigators utilizing these strains, our laboratory processes tissues from representative offspring to ultrapure DNA. This material is then used in DNA fingerprinting with multilocus probes to confirm the results of routine genetic monitoring using isozyme analysis. In addition to the data maintained on rats and mice, we have also demonstrated that hamsters, guinea pigs and pigs bred from closed breeding groups could be differentiated by this technique.