Microehrology in DNA-hydrogels for Molecular imprinting

在过去的几年里，DNA已经被证明是一种材料，可以建造几乎任何纳米尺寸的结构，从定义明确的薄片到电线和有限大小的3D积木。在这个项目中，学生将使用一些公认的DNA结合规则来构建一类新的具有明确结构的三维DNA水凝胶。这涉及到这样一个事实，即我们可以与不同的单链(Ss)DNA形成3路和4路连接，然后使用这些连接来构建3D结构。目的是建立自由悬挂的单链DNA，它仍然附着在网络上，用于感知未知的DNA片段。一旦这些碎片结合在一起，水凝胶的粘弹性将发生显着变化。为了测试这些变化，学生将建立一个基于光学捕获探测粒子的微型流变仪，其波动将被用来提取网络的粘弹性属性。我们将使用国内开发的算法[2]。Seeman，N.C.自然，421,427(2003)；Rohemund，P.W.K.自然，440,297(2006)；Kershner，R.J.等人。自然纳米技术4,557(2009)；Ke，J.等人。自然化学6,994(2014)[2]柳岛，D.Frenkel，J.Kotar，和E.Eiser，“从微观流变学实时监测复杂模数”，“物理学杂志”：简明。《物质，23,194118》(2011年)

In the past few years DNA has been proven to be a material that allows building almost any nano-sized structures ranging from well-defined sheets, to wires and finite-sized 3D building blocks. In this project the student will use some of the well-established DNA-binding rules to build a new class of 3-dimensional DNA-hydrogels with well-defined structures. These involve the use of the fact that we can form 3-way and 4-way junctions with different strands of single-stranded (ss) DNA and use these then to build 3D structures. Aim is to build in freely dangling ssDNA that is still attached to the network for sensing unknown DNA fragments. Once these fragment bind the viscoelastic properties of the hydrogel will change significantly. In order to test these changes, the student will build up a micro-rheometer based on optically trapping a probe particle, whose fluctuations will be used to extract the viscoelastic properties of the network. We will employ a home-developed algorithm for that [2].Seeman, N. C. Nature 421, 427 (2003); Rothemund, P. W. K. Nature 440, 297 (2006) ; Kershner, R. J. et al. Nature Nanotechnology 4, 557 (2009); Ke, J. et al. Nature Chemistry 6, 994 (2014)[2] T. Yanagishima, D. Frenkel, J. Kotar, and E. Eiser, "Real-time monitoring of complex moduli from micro-rheology", J.Phys.: Condens. Matter, 23, 194118 (2011)