MOLECULAR ANALYSIS RESOURCE FOR PRIMARY HUMAN CANCERS

西德尼·基梅尔癌症中心是一个不断发展的机构，其使命是开展基础生物学研究，以了解癌症的机制，并将实验室的发现转化为患者的治疗。25名教职员工在癌症中心开展研究项目，其中近一半由NCI拨款资助。利用分子解剖中可用的最佳工具，其中一半是由NCI赠款资助的。利用分子解剖中可用的最佳工具，其中一半是由NCI赠款资助的。利用分子解剖中可用的最佳工具，其中一半是由NCI赠款资助的。为了利用在癌症生物学和治疗的分子解剖中可用的最佳工具，提供这些工具的共享资源无论在科学上还是在金钱上都是最有价值的。具体确定和请求的共享资源是肿瘤库和登记处、以激光捕获显微镜为特色的组织分析资源和cDNA阵列资源。肿瘤库和登记设施构成拟议共享资源的其他两个组成部分提供的分子分析的基础。众多研究人员的目标是确定肿瘤细胞的分子特性，包括肿瘤进展的特定阶段，并比较相同和不同类型的不同肿瘤的特性。直到最近，根据形态学从肿瘤标本中评估个别选定的细胞或特定的细胞团的能力一直是极其困难的。克服这些困难的最新进展是激光捕获显微解剖仪器的发展。这种仪器允许对目视识别和选择的细胞进行各种类型的分子分析。这些分析中最强大的一种是利用技术来定义差异基因表达，这是SKCC研究人员开创的一个主题，也是SKCC研究的主要优势和兴趣之一。因此，肿瘤登记与激光捕获显微切割和差异基因表达的cDNA阵列分析相结合，为SKCC研究人员探索癌细胞的分子和遗传特性提供了一套强大的共享资源。我们的研究人员将这些服务应用到他们的研究中，证明了他们对这些服务的需求。使用这些共享资源的主要目标是为临床应用确定新的诊断和治疗目标，并更好地了解癌症的发展和转移进展的过程。

The Sidney Kimmel Cancer Center is a growing institution with the mission to conduct basic biological research to under the mechanisms of cancer and to translate discoveries in the laboratories to the treatment of patients. Twenty-five faculty members conduct research programs at the Cancer Center almost half of whom are funded by NCI grants. To utilize the best tools available in the molecular dissection of half of whom are funded by NCI grants. To utilize the best tools available in the molecular dissection of half of whom are funded by NCI grants. To utilize the best tools available in the molecular dissection of half of whom are funded by NCI grants. To utilize the best tools available in the molecular dissection of cancer biology and therapy, shared resources which supply these tools are by far the best value both scientifically and monetarily. The shared resources specifically identified and requested are a tumor bank and registry, a tissue analysis resource featuring Laser Capture Microscopy and a cDNA array resource. The tumor bank and registry facility forms the foundation for the molecular analyses provided by the other two components of the proposed shared resources. The goal of numerous investigators here is to characterize the molecular properties of tumor cells, including specific stages of tumor progression, and to compare the properties of different tumors of the same and dissimilar types. The ability to evaluate individually selected cells or specific clusters of cells from a tumor specimen based on morphology has been extremely difficult until recently. The recent advance that has overcome these difficulties is the development of the laser capture microdissection instrumentation. This instrumentation allows various types of molecular analyses on visually identified and selected cells. One of the most powerful of these analyses utilizes technologies to define differential gene expression, a subject pioneered by SKCC investigators and one that represents a major strength and interest of SKCC research. Thus, the tumor registry coupled with laser capture microdissection and cDNA array analysis of differential gene expression provides a powerful set of shared resources for SKCC investigators to probe the molecular and genetic properties of cancer cells. The need of these services by our investigators is evidenced by their applications of these services to their research Major goals of the use of these shared resources are the identification of new diagnostic and therapeutic targets for clinical application and a better understanding of the processes of cancer development and metastatic progression.