Using mathematical modelling of the Langerhans cell tolerogenic gene regulatory network to identify key molecular regulators of the tolerogenic progra

技能优先级调整：定量生物学假说LC致耐受性能力受一个独特的基因调控网络（GRN）调控，该网络诱导与致耐受性LC程序相关的独特基因表达谱。目的1）研究LC群体内的细胞间变异。2）剖析与LC耐受性相关的独特基因表达谱，并鉴定调节LC耐受原性功能所必需的TF。3）创建LC耐受原性GRN的数学模型，用于定量计算机模拟，其正确预测耐受原性输出基因的表达4）确定调节耐受性应答的关键分子开关并进行体外验证。

Skills Priority Alignment: Quantitative BiologyHypothesisLC tolerogenic ability is regulated by a unique gene regulatory network (GRN), which induces a distinct gene expression profile associated with the tolerogenic LC programme. Aims 1) Investigate cell-cell variation within LC populations.2) Dissect a unique gene expression profile associated with tolerance in LCs and identify the TFs essential for regulating LC tolerogenic function.3) Create a mathematical model of the LC tolerogenic GRN for quantitative in silico simulations that correctly predict the expression of tolerogenic output genes4) Identify the key molecular switches regulating tolerogenic responses and validate in vitro.