TYPE 8 CAPSULE OF STAPHYLOCCUS AUREUS

描述（改编自申请人摘要）：本提案旨在 继续李博士对S.金黄色葡萄球菌8型芙 多糖（CP 8）。 目的：1）研究CP 8基因的调控 表达对环境因素的响应; 2）表征 cap 8基因的功能;和3）确定微囊的作用 在毒力方面。 为了研究CP 8基因的调控，PI将使用 基因融合的缺失和诱变分析，以确定哪个启动子 序列是基因调控所必需的。 他将克隆调控基因 对不同的环境刺激做出反应的基因 方法和转座子诱变。 克隆的基因将被灭活 通过等位基因置换来研究其调控。 他将研究CP 8基因 由全球监管机构agr监管。 研究CP 8的功能 他将使用已经产生的化学突变体。 每个 通过分析基因的积累，将基因与功能联系起来 由特定基因突变引起的中间体。 重点将放在 参与链长调节、运输和聚合的基因。 为了明确微囊在发病机制中的作用，同基因缺失 突变体将在体外吞噬试验中进行测试， 致命性研究

DESCRIPTION (Adapted from the applicant's abstract): This proposal is aimed at continuing Dr. Lee's genetic analysis of the S. aureus type 8 capsular polysaccharide (CP8). The aims are: 1) to study the regulation of CP8 gene expression in response to environmental factors; 2) to characterize the function of the cap8 genes; and 3) to define the role of the microcapsules in virulence. To study the regulation of CP8 genes, the PI will use deletion and mutagenesis analyses of gene fusions to define which promoter sequences are required for gene regulation. He will clone regulatory genes that respond to different environmental stimuli by a reverse genetic approach and by transposon mutagenesis. The cloned gene will be inactivated by allelic replacement to study the regulation. He will study how CP8 genes are regulated by agr, a global regulator. To study the functions of CP8 genes, he will use chemical mutants that have already been generated. Each gene will be correlated with function by analyzing the accumulated intermediate resulting from a specific gene mutation. The focus will be on genes involved in chain-length regulation, transport, and polymerization. To define the role of microcapsules in pathogenesis, isogenic deletion mutants will be tested in an in vitro phagocytic assays and by mouse lethality studies.