CORE--SHARED VECTOR FACILITY

向量技术核心将为 基因转移所需试剂的生产与鉴定 哈佛大学基因转移项目中的每一个项目 治疗心脏、肺脏和血液疾病。除了即时服务外 与向量的产生和分配有关的函数 该计划中的调查人员，核心被设计为高度 灵活性，以便能够迅速带来新的基因转移 由隶属于该计划的调查人员开发的技术，以及 制定必要的方法，以便能够迅速传播 将这些技术带回各个项目的调查人员手中。这个 核心的即时能力将包括瞬时生产 常规逆转录病毒和慢病毒载体的高滴度库存 拥有多种宿主范围，产生稳定的逆转录病毒 使用多个第三和第四个载体生产细胞系 代包装细胞系，生产高度集中 逆转录病毒/VSV G伪型及标准AAV载体的制备 以及第二代或第三代腺病毒载体。除 除了生产病媒，核心还将提供专业知识 用于获得调控基因的载体的设计和构建 表达插入的基因，通过使用组织特异性的 转录调控元件或系统，涉及调控 通过外源方式转录。理查德·穆利根博士是一位 哺乳动物载体和包装细胞系的发展，将 担任核心董事。

The Vector Technology Core will provide a centralized service for the production and characterization of gene transfer reagents necessary for each of the individual projects in the Harvard Program in Gene Transfer for Heart, Lung and Blood Disease. In addition to its immediate service functions related to the production and distribution of vectors to investigators in the Program, the Core is designed to be highly flexible, so as to be able to rapidly bring in new gene transfer technologies developed by investigators affiliated with the Program, and develop the methodology necessary to permit the rapid dissemination of those technologies back to investigators in the individual projects. The immediate capabilities of the Core will include the transient production of high titer stocks of conventional retroviral and lentivirus vectors possessing a variety of host ranges, the generation of stable retroviral vector producing cell lines using a number of third and fourth generation packaging cell lines, the production of highly concentrated retroviral/VSV G pseudotypes, and the production of standard AAV vectors and second or third generation adenoviral vectors. In addition to the production of vectors, the Core will also provide expertise in the design and construction of vectors useful for obtaining the regulated expression of inserted genes, either through the use of tissue-specific transcriptional control elements or systems involving the regulation of transcription by exogenous means. Dr. Richard Mulligan, an expert in the development of mammalian vectors and packaging cell lines, will serve as the Director of the Core.