DEVELOPMENT OF 3D DIGITAL HOLOGRAPHY FOR TRACKING MOSQUITO FLIGHT 1=Engineering 2=Sensors and Instrumentation

在大体积、房间大小的空间上进行定量、高动态范围的3D成像面临着根本的挑战。这个项目是由我们的团队与利物浦热带医学院(LSTM)合作开展的，目的是量化蚊子的行为，以设计新的、新的干预措施来减少传染病的传播。据我们所知，我们是目前唯一一个如此全面和详细地研究蚊子体内空间活动的小组。威斯康星大学光学工程师和LSTM媒介生物学家之间的合作始于2011年，并开发了独特的视频跟踪系统，使人们能够前所未有地表征按蚊。和库蚊(Culex sp.)蚊子在驱虫蚊帐中寻找寄主的行为。我们的研究方法是独特的，允许对媒介行为进行研究，这在以前是不可能的，并且已经被证明是一条快速和富有成效的新工具的途径。这两个小组有7个项目获奖的联合记录(目前有5个)。目前的活动主要是应用研究，涉及光学成像和数据分析，以得出自然行为。这项博士项目计划旨在研究一种更具挑战性的基于数字全息术(DH)的3D成像方法，以克服现有方法的局限性：获得的3D位置信息的准确性、现场实施的简便性、能够测量更大的体积和通过蚊帐材料成像。水声是一种相对较新的成像方式，在这种方式下，从场景散射的复杂波前在数字探测器上以干涉方式记录下来。使用计算方法将波前反向传播到测量体积内的感兴趣平面，从而能够重建相位和幅度。大部分成功申请卫生署服务的个案都是在微米至毫米的水平。这项计划将探讨将水解度的动态范围扩大到微米级分辨率的米级体积。检测散射场的相位的能力有望在蚊子成像中特别有用，这将产生特征相位扰动，该特征相位扰动可用于指导重建算法识别单个蚊子的平面，从而识别3D位置。研究成果将发表在高级光学工程期刊上，如《光学快报》、《光学快报》和《应用光学》。LSTM已证实，他们乐于支持在其杀虫器中试验该仪器，以检查蚊子接近蚊帐缺陷的详细行为，以及新型蚊子捕蚊器的设计。因此，可以预期在排名更高的期刊上发表更多的文章。我们现有的项目将酌情提供一个在该领域进行开发的平台。

There are fundamental challenges to quantitative, high dynamic range 3D imaging over large volumes, room sized spaces. This project is motivated by our groups work in quantifying mosquito behaviour in order to design novel and new interventions to mitigate the spread of infectious disease which is a collaboration with Liverpool School of Tropical Medicine (LSTM). To our knowledge, we are the only group currently investigating in vivo spatial activity of mosquitoes at such a comprehensive and detailed level. The collaboration between UoW optical engineers and LSTM vector biologists started in 2011 and has developed unique video tracking systems that have enabled unprecedented characterisation of Anopheles sp. and Culex sp. mosquito host-seeking behaviour at insecticide treated bed nets. Our research approach is distinctive, permitting investigation of vector behaviours not previously possible, and already shown to be a rapid and productive route to new tools. The groups have a joint track record of 7 project awards (5 current). The current activities are largely applied research involving optical imaging and data analytics to derive natural behaviours. This PhD project proposal seeks to investigate a more challenging 3D imaging methodology based on digital holography (DH) to overcome the limitations of existing approaches: accuracy of the 3D positional information obtained, ease of implementation in the field, ability to measure over an extended volume and to image through bednet materials. DH is a relatively new imaging modality in which the complex wavefront scattered from a scene is recorded interferometrically at a digital detector. Computational methods are employed to back propagate the wavefront to planes of interest within the measurement volume enabling reconstructions of both phase and amplitude. The majority of successful DH applications have been at the micron to millimetre scale. This project will explore extending the dynamic range of DH to metre scale volumes within micron scale resolution. The ability to detect the phase of the scattered field is expected to be particularly helpful in imaging mosquitoes which will generate a characteristic phase perturbation which can be used to guide the reconstruction algorithms to identify the plane, and hence 3D position, of individual mosquitoes. The outputs of the research will be reported in high ranking optical engineering journals, such as Optics Express, Optics Letters and Applied Optics. LSTM have confirmed that they are happy to support trials of the instrument in their insectaries to examine, for example, detailed behaviour of mosquitoes approaching faults in bednets and the design of novel mosquito traps. Hence, further publications can be expected in higher ranking journals. Our existing projects will provide a platform for exploitation in the field as appropriate.