REGULATION OF FUNCTIONAL RECOVERY AFTER SPINAL INJURY

描述：(逐字摘自申请者的摘要)七鳃鳗幼体 脊髓损伤后轴突再生和功能恢复 损伤。然而，PI发现，通过改变温度， 动物被饲养，就有可能生产出表现出 功能性或功能失调的游泳行为。因此，七鳗鱼提供了 有机会研究功能失调的再生的性质和后果。 她进一步发现了再生系统之间的解剖学差异 在这两个条件下，特别是在分配方面 5-羟色胺能轴突。在横断面之后，有一个戏剧性的增加 5-羟色胺纤维位于病变吻部区域，尾部明显缺失。 细分市场。然而，在有利于恢复的条件下，5-羟色胺纤维 更多的是在尾部。她假设5-羟色胺纤维 至少有一种形式的行为差异是由 不同的温度条件。 PI现在建议确定升高的5-羟色胺的来源 吻段的神经支配和尾部丢失的5-羟色胺的命运 细分市场。将被探索的一种可能性是，尾部的增加 节段是由于5-羟色胺系统下行的锁骨萌发所致。她 将通过评估5-羟色胺纤维是否起源于 来自脑干或背根。多巴胺纤维的命运也将 被评估。其他研究将评估该系统的功能 动物使用复杂的随机阶段模型来评估生理 回应。这项由PI开发的技术提供了一个 对分段之间协调度的定量测量。在 同样的动物，再生的纤维将被解剖并使用 GABA和甘氨酸的特异性标志物。

DESCRIPTION: (Verbatim from the Applicant's Abstract) The larval lamprey exhibits axonal regeneration and functional recovery following spinal cord lesions. However, the PI has found that by altering the temperature at which animals are maintained, it is possible to produce animals that exhibit either functional or dysfunctional swimming behavior. Thus the lamprey offers the opportunity to study the nature and consequences of dysfunctional regeneration. She has further found anatomical differences between the regenerated systems under the two conditions, specifically in terms of the distribution of serotoninergic axons. Following transections, there is a dramatic increase in serotonin fibers in areas rostral to the lesion, and a dramatic loss in caudal segments. However under conditions that favor recovery, serotonin fibers are more abundant in the caudal segments. She hypothesizes that serotonin fibers are responsible for at least one form of the behavioral differences seen under the different temperature conditions. The PI now proposes to identify the sources of the increased serotonin innervation in rostral segments, and the fate of the lost serotonin in caudal segments. One possibility that will be explored is that the increases in caudal segments is due to collareral sprouting of descending serotonin systems. She will test this hypothesis by evaluating whether the serotonin fibers originate from the brainstem or from dorsal roots. The fate of dopamine fibers will also be evaluated. Other studies will evaluate the functional capabilities of the animals using a sophisticated stochastic phase model to evaluate physiological responses. This technique, which has been developed by the PI, provides a quantitative measure of the degree of coordination between segments. In the same animals, the regenerated fibers will be traced anatomically and using specific markers for GABA and glycine.