CORE--MICROSCOPY LABORATORY

在过去的10年里，随着新的显微镜形式和样品制备程序的发展，显微镜领域取得了重大进展。我们为癌症研究人员提供了使用特定抗体或标记的互补核酸序列来专门标记蛋白质、碳水化合物和核酸序列的能力。我们还可以方便地使用荧光分子来定位特定的细胞结构、蛋白质和核酸，以及观察和测量特定细胞器的生理变化。随着核心中使用的显微镜的种类和复杂程度的增加，主任Jay Jerome博士和核心技术人员的专业知识所增加的价值成为一个重要组成部分，使经验丰富的研究人员能够利用这些工具，并有效地向新手用户建议哪种技术最适合他们的研究。该设施拥有一个全方位的准备实验室，配备了标准的广场荧光显微镜，包括飞利浦400透射式(120keV)电子显微镜、飞利浦515分析扫描电子显微镜、飞利浦CM-30中压(300keV)透射式电子显微镜、蔡司510激光扫描共聚焦显微镜、配备视频和数字成像功能的蔡司直立和倒置广场显微镜，以及Arcturus PixCell II激光解剖显微镜。蔡司倒置显微镜配备了一个温度和环境控制室，与蔡司倒置显微镜一起使用，从而允许在受控的二氧化碳环境中进行长期的活细胞实验。对这个核心的资助与对其他核心的资助略有不同。根据定义，癌症中心批准的项目使用CCCWFU资助的显微镜核心实验室部分是100%的，因为支持是以按存储容量使用补充的形式提供的，这只给予获得批准的癌症重点资助的项目。在过去的两年里，由于目前的提案中癌症中心的规模大幅缩小，我们要求提供20,516美元，作为实验室对CCWFU成员项目的服务费的补充。

The last 10 years have seen major advances in the field of microscopy with the development of new forms of microscopy and specimen preparation procedures. We have available to cancer investigators the ability to specifically tag proteins, carbohydrates, and nucleic acid sequences using specific antibodies or labeled complementary nucleic acid sequences. We can also facilitate the use of fluorescence molecules for localizing specific cellular structures, proteins and nucleic acids, and for viewing and measuring physiological alterations in specific organelles. With the increased variety and sophistication of microscopes utilized in the Core, the value added by the specialized knowledge of the Director Dr. Jay Jerome and the Core technicians becomes an essential component, enabling seasoned investigators to utilizes the tools and efficiently advising novice users on which of the variety of techniques is most appropriate to their research. The facility maintains a full service preparative laboratory and houses standard wide field fluorescent microscopes a Philips 400 Transmission (120 KeV) Electron Microscope, Philips 515 Analytical Scanning Electron Microscope, Philips CM-30 Intermediate Voltage (300 KeV) Transmission Electron Microscope, Zeiss 510 Laser Scanning Confocal Microscope, Zeiss upright and inverted widefield microscopes equipped for video and digital imaging, and a Arcturus Pixcell II Laser Dissection Microscope. The Zeiss inverted microscope is equipped with a temperature and environment controlled chamber for use with the Zeiss inverted microscope This allows along term, live cell experiments in a controlled CO2 environment. Funding of this Core is slightly different from the funding of other Cores. Usage of the CCCWFU-funded portion of the Microscopy Core Laboratory by Cancer Center approved project is by definition 100% since support is in the form of a charge-back supplement, which is only given to approved cancer-focused, funded projects. Over the past two years, due to the substantial reduction in the size of the Cancer Center in the current proposal we are requesting $20,516 which will be used as a supplement for fee for service charge by the laboratory to projects of CCWFU membership.