Core--Gene expression vector

基因表达载体核心（GEVC）将为Nathan Shock Center附属研究人员提供最先进的分子生物学资源，以促进他们对正常衰老和年龄相关疾病病理生理学的研究。GEVC将协助开发和生产基于单纯疱疹病毒扩增子载体的基因转移试剂。它们适用于体外和体内基因转移研究。此外，GEVC将使用“实时”PCR进行定量核酸测量。虽然扩增子载体已被证明是一种非常有效的载体，用于将遗传物质递送到几种动物物种的多种组织和细胞类型，但其对老龄化社区研究人员的可及性将促进现有研究并实现新的研究。类似地，“实时”定量PCR技术已经提供了用于定量来自细胞和组织的特定核酸序列（RNA或DNA）的可再现和高通量方法。然而，由于与初始设备成本有关的大量费用，大多数调查人员无法利用这一宝贵的技术。通过利用罗切斯特大学现有的专业知识和设施，GEVC旨在为当地、地区和国家的休克中心研究人员以及其他研究人员提供基于扩增子载体的基因转移和定量PCR技术。GEVC将被安置在衰老和发育生物学中心内新建的亚瑟科恩伯格医学研究大楼的一楼。GEVC将由William J. Bowers博士指导，霍华德J. Federoff博士共同指导，而日常核心活动将由实验室技术助理执行。GEVC科学监督委员会将由共同主任和John Olschowka博士（定量PCR顾问）组成。

The Gene Expression Vector Core (GEVC) will provide Nathan Shock Center-affiliated investigators with state-of-the-art molecular biological resources that facilitate their studies of normal aging and pathophysiology of age-related disease. The GEVC will assist in the development and production of gene transfer reagents based on the herpes simplex virus- based amplicon vectors. These are suitable for in vitro and in vivo gene transfer studies. In addition the GEVC will perform quantitative nucleic acid measurements using "real-time" PCR. While the amplicon vector has been shown to be a very effective vehicle for the delivery of genetic material to numerous tissue and cell types of several animal species, its accessibility to investigators in the aging community will facilitate existing studies and enable new ones. Similarly, the "real-time" quantitative PCR technology has provided to be a reproducible and high throughput method for quantitation of specific nucleic acid sequences (RNA or DNA) from cells and tissues. However, due to the large expense related to initial equipment costs, most investigators are unable to utilize this valuable technology. By taking advantage of the expertise and facilities that already exists at the University of Rochester, the GEVC seeks to provide both amplicon vector-based gene transfer and quantitative PCR technologies to Shock Center investigators locally, regionally and nationally and other researchers pursuing aging-related problems. The GEVC will be housed on the first floor of the newly constructed Arthur Kornberg medical Research Building within the Center for Aging and Developmental Biology. The GEVC will be directed by Dr. William J. Bowers and co-directed by Dr. Howard J. Federoff and, while day-to-day core activities will be performed by a laboratory technical associate. The GEVC scientific oversight committee will consist of the co-directors and Dr. John Olschowka (quantitative PCR consultant).