MOLECULAR BASIS FOR VERTEBRATE CELL MOTILITY

这位研究人员建议分析两种肌动蛋白结合蛋白，即 原肌球蛋白异构体和钙调蛋白对非肌肉细胞的调节 能动性。具体地说，(1)非肌肉原肌球蛋白亚型在 胞质分裂将被确定；(2)分子的影响 钙调蛋白对微丝稳定性和运动性的影响 将研究突变体的行为；(3)原肌球蛋白的作用 胞内颗粒运动中的异构体和钙调蛋白 通过分析原肌球蛋白亚型和原肌球蛋白异构体的相互作用进行分析 钙调蛋白对肌动蛋白和肌球蛋白I及肌动蛋白和肌球蛋白相互作用的影响 V.还(4)原肌球蛋白异构体的调节将扩大到 果蝇模型与果蝇转运和定位的关系 胚胎发育过程中的母性信息。一种分子的组合 生物学、细胞生物学、生化和免疫学方法 将用于研究上述蛋白质的作用。

The investigator, proposes to analyze two actin binding proteins, the tropomyosin isoforms and caldesmon in regulating nonmuscle cell motility. Specifically (1) the role of nonmuscle tropomyosin isoforms in cytokinesis will be ascertained; (2) the effects of molecular manipulation of caldesmon on microfilament stability and motile behavior in mutants will be studied; (3) the role of tropomyosin isoforms and caldesmon in intracellular granule movements will be analyzed, by analyzing interaction of tropomyosin isoforms and caldesmon on interactions of actin and myosin I and actin and myosin V. Also (4) the regulation of tropomyosin isoforms will be extended to the Drosophila model in relation to transport and localization of maternal messages during embryogenesis. A combination of molecular biological, cell biological, biochemical; and immunological approaches will be used to study the role of the aforementioned proteins.