CORE--METABOLISM LABORATORIES

核心单元的目标是提供持续的高质量 为PERC中的代谢研究提供分析支持。 该目的可以 以具有成本效益的方式有效实现， 一些调查人员使用的中央核心设施。 这 对于稳定同位素和质谱分析特别必要， 由于方法的复杂性和高成本， 购买和维护必要的设备。 过去 几年来，这些目标都已顺利实现。 核心代谢实验室已经产生了重大影响， 生产力和研究质量的各种调查员在 PERC。 GC-MS实验室开发了新的创新稳定同位素 用于研究妊娠受试者的代谢的方法和 新生儿 下列稳定同位素的分析技术 在我们的实验室中建立了标记底物：葡萄糖，尿素， 丙氨酸，亮氨酸，赖氨酸，丝氨酸和甘氨酸，乳酸，棕榈酸， 甘油和酮。 所有的方法现在都是常规分析。 在 此外，还介绍了测量13 C富集度的分析技术 血液中的碳酸氢盐已经被提炼出来了 最近增加的是α-酮酸的定量分析 支链氨基酸的GC-MS和HPLC。 这些技术 重要的是，为了测量前体池富集， 定量蛋白质合成。 所提供的服务将包括样品分析，帮助解释， 监督和咨询。 核心还负责 维护和适当运作的复杂的分析 仪器. 所有使用稳定同位素的代谢方案 追踪器将使用核心实验室

The objective of the Core unit is to provide continued high quality analytical support for the metabolic studies in the PERC. This aim can be achieved efficiently and in a cost-effective way, by having a centralized Core facility utilized by a number of investigators. This is particularly necessary for stable isotope and mass spectrometry, because of the complicated nature of methods and the high cost of purchasing and maintaining the necessary equipment. Over the past several years, all of these objectives have been successfully achieved. The Core Metabolism Laboratories have had a major impact on the productivity and the quality of research of various investigators in the PERC. The GC-MS Laboratory has developed new innovative stable isotope methods for the study of metabolism in the pregnant subject and the newborn infants. Analytical techniques for the following stable isotope labelled substrates are established in our laboratories: glucose, urea, alanine, leucine, lysine, serine and glycine, lactate, palmitic acid, glycerol, and ketones. All of the methods are now routine analysis. In addition, the analytical technique for the measurement of 13C enrichment of blood bicarbonate has been refined. A more recent addition has been the quantitative analysis of a-keto acids of branched-chain amino acids on GC-MS & HPLC. These techniques were important in order to measure the precursor pool enrichment for quantifying protein synthesis. Services provided will include sample analysis, help in interpretation, supervision and consultation. The Core is also responsible for the maintenance and adequate functioning of the sophisticated analytical instruments. All of the metabolism protocols utilizing stable isotope tracers will use the Core Laboratory.