NMR Studies of Phoshate Metabolism in Protozoan Parasite

描述（由申请人提供）：本研究的目的是使用高 场核磁共振光谱研究代谢 克氏锥虫中发生的过程，并利用这些信息来帮助 发现新的药物靶点将采用的方法进一步 克氏锥虫代谢过程的研究涉及使用600和 750 MHz溶液和固态NMR研究31 P和13 C NMR光谱 上鞭毛体和无鞭毛体两种形态的迄今为止，最初的31 P和13 C NMR 光谱已经获得，但碳水化合物和 磷酸盐代谢，氨基酸和磷酸盐代谢， 研究了因此，下一步需要做的是， 碳和磷酸盐代谢之间的相关性，这项工作似乎是 由于发现了非常高水平的焦磷酸盐，这是必不可少的。也就是说， 焦磷酸盐的作用需要澄清。在这里，能够监控 可溶性缩合磷酸盐（使用溶液NMR）以及不溶性 磷酸盐（使用固态魔角样品旋转技术）应 证明是无价的此外，溶液和固态核磁共振技术将用于 研究在T cruzi中发现的不寻常的聚-a-羟基辛酸酯物种， 通过与发现于 原核生物可能代表酸钙体中的原始离子通道。

DESCRIPTION (provided by applicant): The aim of this research is to use high field nuclear magnetic resonance spectroscopy to investigate the metabolic processes that occur in Trypanosoma cruzi and to use this information to help discover new drug targets. The methods to be employed for the further investigation of the metabolic processes in T cruzi involve the use of 600 and 750 MHz solution and solid-state NMR to study the 31P and 13C NMR spectroscopy of both epimastigote and amastigote forms. To date, initial 31P and 13C NMR spectra have been obtained, but the relationships between carbohydrate and phosphate metabolism, and amino acid and phosphate metabolism, have not been investigated. What needs to be done next, therefore, is to make the first correlations between carbon and phosphate metabolism, work that appears to be essential due to the very large levels of pyrophosphate found. That is, the role of pyrophosphate needs to be clarified. Here, the ability to monitor both soluble condensed phosphates (using solution NMR) as well as insoluble phosphates (using solid-state magic-angle sample-spinning techniques) should prove invaluable. Plus, solution and solid-state NMR techniques will be used to investigate the unusual poly-a-hydroxyoctanoate species found in T cruzi, which by analogy with the polyphosphate/poly-cx-hydroxybutyrate species found in prokaryotes may represent a primitive ion channel in the acidocalcisome.