CORE--BIOLOGICAL IMAGING ANALYSIS CORE

我们请求支持生物成像核心， 履行与本PPG相关的项目的基本职能 应用程序.根据PPG成员取得的显著进展， 在过去的赠款期间，很明显， 该提案中的所有项目现在都已准备就绪， 形态学的相关性对于将生物化学和遗传学 信息到一个结构化的背景下。生物成像核心 目的如下：第一，核心将提供技术支持 所需的所有方面的准备，查看和解释薄 切片电镜。EM使用的一个主要方面是 抗原免疫细胞化学定位技术的可用性 使用嵌入前免疫金标记和嵌入后定位 冷冻薄片和亲水性塑料切片上。二是 核心将支持光学显微镜免疫细胞化学的各个方面 这是分析细胞中表达的抗原的关键技术。 Core将为BioRad MRC 600的使用提供实际帮助 激光扫描共聚焦显微镜，并将继续帮助 标签抗原和其他抗原检测程序的开发 活细胞中的荧光探针。图像分析的各个方面， 重建、数字化定量分析和数据显示， 出版前的准备工作可通过核心获得。最后通过一个 核心意志的一个重要方面是为互动提供一个焦点 在本PPG的研究者中， 计划、执行和解释实验， 合作。 博士Jamieson将担任该矿石的总经理;日常 核心的操作将由保罗韦伯斯特博士进行，他是 熟悉材料制备和分析的各个方面， 光镜和电镜免疫细胞化学。他将监督工作流程， 琳达奇乔内女士协助下， 协助样品制备和暗室工作。菲利普·马莱先生将 提供共聚焦系统使用方面的工程支持和培训 和电子显微镜。所有与核心有关的专业人员都是 可就试验性办法、技术咨询 以及PPG中大多数项目的合作者。最后 工作人员的主要职责是培训、监督和 质量控制的新的学生和博士后研究员谁将 参与PPG项目。

We are requesting support for a Biological Imaging Core that will continue to perform an essential function for the projects associated with this PPG application. Based on the remarkable progress made by members of the PPG during the past grant period, it is clear that the research activities of all of the projects in this proposal are now poised at a point where morphological correlates are essential for placing biochemical and genetic information into a structural context. The Biological Imaging Core's prime purposes are as follow: First, the Core will provide technical support required for all aspects of preparation viewing and interpretation of thin section electron microscopy. A major aspect of EM use will be the availability of techniques for immunocytochemical localization of antigens using pre embedding immunogold labeling and post embedding localizations on frozen thin sections and sections of hydrophilic plastics. Second, the Core will support all aspects of light microscopic immunocytochemistry which is a critical technique for analysis of antigens expressed in cells. The Core will provide practical help with the use of the BioRad MRC 600 laser scanning confocal microscope and will continued to aid in the development of procedures for examination of tagged antigens and other fluorescent probes in live cells. All aspects of image analysis and reconstruction, quantitative analysis of digitized and data display and preparation for publication are available through the Core. Finally, an important aspect of the Core will to provide a focus for interaction amongst investigators in this PPG and to significantly aid in the planning, execution and interpretation of experiments through collaborations. Dr. Jamieson will serve as overall director of this ore; day to day operation of the Core will be carried out by Dr. Paul Webster who is conversant in all aspects of the preparation and analysis of material for light and EM immunocytochemistry. He will oversee the flow of work through the Core, assisted by Ms. Linda Chicione who provides general technical help with specimen preparation and darkroom work. Mr. Philippe Male will provide engineering support and training in the use of the confocal system and electron microscopes. All professionals associated with the Core are available for consultation on experimental approaches, technical advice and for collaborators on most of the projects in the PPG. Finally, a central responsibility of the staff will be training, supervision and quality control of new students and postdoctoral fellows who will participate in PPG projects.