IMMUNE PEPTIDES IN AEDES AEGYPTI

描述(改编自申请人的摘要)：本提案来自 一位新的调查者正致力于描述4 来自免疫激活的Ae.埃及伊蚊。使用MALDI-TOF MS与P.Bulet(法国斯特拉斯堡CNRS)合作，PI有 获得了在Ae中至少有15个免疫诱导多肽的证据。 埃及伊蚊。初步数据支持对天蚕素(CEC)的分析 对富含脯氨酸的革兰氏阴性细菌(GR)有活性的蛋白质 多肽(PRO)和一个690Da分子(M690)。编码这四种基因 分子将被克隆，使用聚合酶链式反应和相关技术， 将测定多肽的抗菌谱。最后， 将使用Sindbis载体在蚊子中表达蛋白质，并且 蚊子随后会接触到一种具有传染性的血粉。 (鸡疟原虫和马来丝虫)，以确定其作用 这些多肽，单独或组合在一起，对寄生虫的发育起作用。

DESCRIPTION (Adapted from the Applicant's Abstract): This proposal from a new investigator is directed towards the characterization of 4 molecules from immune-activated Ae. aegypti mosquitoes. Using MALDI-TOF MS in collaboration with P. Bulet (CNRS, Strasbourg, France), the PI has obtained evidence for at least 15 immune-inducible peptides in Ae. aegypti. Preliminary data support the analysis of a cecropin (CEC), a protein active against Gram negative bacteria (GR), a proline-rich peptide (PRO) and a 690 Da molecule (M690). Genes encoding these four molecules will be cloned, using PCR and related technologies, and the antimicrobial spectrum of the peptides will be determined. Finally, the proteins will be expressed in mosquitoes using a Sindbis vector, and the mosquitoes will subsequently be exposed to an infectious bloodmeal (Plasmodium gallinaceum and Brugia malayi) to determine the effects of the peptides, alone and in combination, on parasite development.