The role of Prop1 in pituitary cell proliferation

脑下垂体的发育依赖于周围组织产生的外在因子和脑下垂体固有的转录因子在空间和时间上的适当表达。这些信号用于控制脑下垂体前叶5种激素产生细胞类型的规格和增殖。由脑下垂体发育缺陷引起的一种常见疾病是合并脑下垂体激素缺乏症(CPHD)。已知在这种疾病中发生突变的一个基因是Prop1。患者和Propl基因突变的小鼠的血清激素促甲状腺激素、生长激素、催乳素和促性腺激素水平降低或可以忽略不计。垂体体积的减少是由于产生这些激素的细胞不能增殖所致。为了明确Propl在垂体细胞增殖中的作用，将进行以下研究：鉴定Prop1持续缺失或过度表达的垂体的表型，并验证和鉴定可能的Prop1靶基因。

Development of the pituitary gland relies on the proper spatial and temporal expression of extrinsic factors produced by the surrounding tissue and transcription factors intrinsic to the pituitary. These signals serve to control the specification and proliferation of the 5 hormone producing cell types of the anterior pituitary gland. A common disease that results from a defective pituitary deirelopment is combined pituitary hormone deficiency (CPHD). One gene known to be mutated in this disease is Prop1. Patients as well as mice with mutations in Propl present with decreased or negligible levels of the serum hormones thyrotropin, growth hormone, prolactin and gonadotropins. Decreased pituitary volume results from failure of the cells that produce these hormones to proliferate. To define the role of Propl in pituitary cell proliferation, the following studies will be performed: characterize the phenotype of pituitaries in which Propl is either absent or overexpressed persistently, and validate and characterize putative Propl target genes.