CYTOTOXIC T LYMPHOCYTE ACTIVATION IN VITRO AND IN VIVO

描述：(改编自申请人的摘要) 申请，梅舍尔博士建议继续他的研究，以确定 激活幼稚和记忆所必需的配体 细胞毒性T淋巴细胞。利用他的开创性系统 用于传递定义的信号的人造表面和采用的 从转基因TCR小鼠中转移CTL，梅舍尔博士建议 证实了他在识别关键信号方面所做的大部分前期工作 负责触发CTL激活。这份续期申请是 扩大到包括实验，以确定IL-12的潜在作用 在激活朴素的CTL反应以及调查 炎性细胞因子在影响记忆细胞中的潜在作用 功能。两个具体目标，这两个目标基本上与 原始项目的具体目标，以及重要的添加 提出了可采用的转基因小鼠系统。

DESCRIPTION: (Adapted from the applicant's abstract) In this application, Dr. Mescher proposes to continue his studies identifying ligands that are essential for the activation of naive and memory cytotoxic T lymphocytes. Utilizing both his pioneering system of artificial surfaces for the delivery of defined signals and an adoptive transfer of CTLs from transgenic TCR mice, Dr. Mescher proposes to confirm much of his preliminary work identifying the key signals responsible for triggering CTL activation. This renewal application is expanded to include experiments to define the potential role that IL-12 plays in activating the naive CTL response as well as investigating a potential role for inflammatory cytokines in influencing memory cell function. Two specific aims, which are essentially identical to the specific aims of the original project, with the important addition of the adoptive transgenic mouse system are proposed.