Hydrolase discovery in the extremophile Galdieria

Galdieria是一种红藻，具有多种代谢功能，在高酸性条件下（低至pH 0）和高于55℃（理论上的真核生物极限）的温度下表现出巨大的生存能力。这个属的成员显示出广泛的代谢谱，允许在几乎任何糖、糖醇或有机酸源上旺盛生长，它能够光自养和化学异养生长。Galdieria是新陈代谢的主力。Galdieria的极端生活方式使得寻找具有工业生物技术（IB）应用特性的新物种成为一种迷人的生物。为了开发Galdieria在ib领域的应用，我们必须定义其代谢能力的多样性。在这个项目的第一阶段，学生将进行ib相关酶的系统基因组发现。从那里，注释导致使用利用基因组尺度代谢网络的创新管道对代谢预测的描述。在第二阶段，将对排泄的酶进行信息描述，这些酶可以在非常低的pH值和升高的温度下发挥作用。从分类酶的多样性来看，同源性搜索将用于识别木聚糖酶和其他纤维素酶、蛋白酶和氧化酶。在第三阶段，学生将进行酶的表征，并开发有关工艺生产性能的信息，以达到工业化前的规模扩大。根据定义，所有已鉴定的酶都必须在高温和极低ph下起作用。因此，考虑到Galdieria生活在真核生物的绝对极限，这些酶将是迄今从真核生物中分离出的最耐热和耐酸的酶。

Galdieria is a red alga that exhibits metabolic versatility and displays enormous capacity to thriveat highly acidic conditions (down to pH 0) and temperatures above 55C, the theoretical limit ofeukaryotic life. Members in this genus display broad metabolic repertoires allowing vigorousgrowth on virtually any sugar, sugar-alcohol or organic-acid source and it is capable of bothphotoautotrophic and chemoheterotrophic growth. Galdieria is a metabolic workhorse. Theextremophile lifestyle of Galdieria makes it a fascinating organism to find those novel species withproperties that have industrial biotechnological (IB) applications. To exploit Galdieria for use in theIB sector, we must define the constellation of diversity in metabolic capacity it displays. In phase 1of this project, the student will perform phylogenomic discoveries of IB-relevant enzymes. Fromthere, annotation leads to a description of metabolic predictions using the innovative pipeline thatexploits genome-scale metabolic networks. In phase 2, informatic descriptions will be produced ofexcreted enzymes that can function under very low pH and elevated temperature. From the largediversity of classified enzymes, homology searches will be used to identify xylanases and othercellulases, proteases and oxidative enzymes. In phase 3, the student will perform enzymecharacterisation and develop information on process-production performance to a pre-industrialscale-up. By definition, all identified enzymes must function at high temperature and very low pH.Thus these will be the most heat- and acid-resistant enzymes ever isolated from a eukaryote,given that Galdieria lives at the absolute limits of eukaryotic life.