Role of CIZ1 in X chromosome inactivation

X染色体失活（XCI）是通过多梳抑制复合物（PRC） 1和2的作用，在整个基因组中建立和维持选择性转录沉默的一种模式。核基质（NM）蛋白cip1相互作用锌指蛋白1 （CIZ1）与细胞周期蛋白相关，在DNA复制中起作用，并与成人和儿童癌症有关。我们最近发现，在小鼠和人类女性细胞中，CIZ1位于Xi位点，缺乏CIZ1会导致高度渗透的女性特异性淋巴细胞增生性疾病。数据表明，通过与核基质的关联，在维持X染色体失活中起作用。该项目将研究CIZ1在细胞周期中维持表观遗传标记的机制，并将使用一系列分子和细胞生物学技术，包括高分辨率和实时成像。

X chromosome inactivation (XCI) serves as a model for establishment and maintenance of selective transcriptional silencing throughout the genome,through the action of polycomb repressive complexes (PRC) 1 and 2. The nuclear matrix (NM) protein Cip1-interacting zinc finger protein 1 (CIZ1) plays a role in DNA replication in association with cyclins, and is linked with adult and paediatric cancers. We recently showed that CIZ1 is located at the Xi in mouse and human female cells, and that absence of CIZ1 leads to highly penetrant female-specific lympho-proliferative disorder. The data argue for a role in maintenance of X chromosome inactivation through association with the nuclear matrix. This project will investigate the mechanism by which CIZ1 functions in the maintenance of epigenetic marks as cells pass through the cell cycle, and will use a range of molecular and cellular biology techniques including high resolution and live imaging.