RYANODINE RECEPTOR REGULATION

描述（改编自申请人的摘要）： 提出的研究是为了验证这一假设，即激活快速 通过改变磷酸化水平来调节骨骼肌的抽搐 肌浆网（SR）的Ryanodine受体（RyR）状态 钙释放通道在本项目中，研究人员将使用 反磷酸化-断裂-衍生化（BFD）和 片段化蛋白质印迹（frag-blot）方法来测定体内 不同RyR中特定位点的磷酸化化学计量 在非活化肌肉中以及在强直性脊柱炎期间， 刺激，以及在中度和重度疲劳期间。 大鼠伸肌 趾长肌（EDL）和青蛙半腱肌将进行比较 确定RyR的磷酸化是否在含有RyR的肌肉中不同。 不同比例的RyR亚型。 此外，选定的研究将 扩大到包括兔子和鸡的肌肉，以测试 哺乳动物和非哺乳动物物种之间的种间变异。

DESCRIPTION (Adapted from the Applicant's Abstract): The goal of the proposed studies is to test the hypothesis that activation of fast twitch skeletal muscles is regulated by altering the phosphorylation state of the ryanodine receptor (RyR) sarcoplasmic reticulum (SR) calcium release channels. In this project the investigator will use the back phosphorylation-fragmentation-derivatization (BFD) and fragmentation Western blot (frag-blot) methods to determine the in vivo phosphorylation stoichiometries of specific sites in the different RyR isoforms in vivo in nonactivated muscles, as well as during tetanic stimulation, and during moderate and severe fatigue. Rat extensor digitorum longus (EDL) and frog semitendinosus muscles will be compared o ascertain if phosphorylation of the RyRs differ in muscles containing different ratios of RyR isoforms. In addition, selected studies will be expanded to include rabbit and chicken muscles to test for interspecies variations between mammalian and nonmammalian species.