Composition/Functional Elasticity of Engineered Tissues

描述：(申请人提供)组织工程学是一个新兴领域 牙齿和其他颅面特征的重建外科手术包括 皮肤、骨头或软骨。在一种常见的组织修复方法中，细胞 能够增殖和合成细胞外基质(ECM)蛋白 种植在三维可生物降解的聚合物支架上。脚手架 为结构提供机械稳定性和几何形状，直到 细胞和生化成分发育成有能力的组织。在 工程组织的设计，平衡生物材料的过程 伴随着组织合成的退化在愈合的最初阶段是关键的 以及长期的成功。这两个过程的结合提供了质量 和材料的体积平衡，旨在保持 修复组织的生物和机械健康。这些相辅相成， 依赖时间的过程通常仅通过经验来定义，因为缺乏 已建立参数关系。据推测，数学上的 可以建立模型来描述伴随物的动态质量状态 支架退变和组织合成。该模型可以在实验上进行 验证以确认构成质量并预测功能弹性 工程化组织复合材料的各项参数。该模式还将提供 用于优化开发的设计标准和性能规范 体内生物和机械环境中的工程化组织。它 旨在使此项目中定义的一般关系可以是 针对所有工程组织类型进行了修改。在下面的提案中，有两个 将开发动态质量模型(聚合物和ECM质量)以定义 建立软骨的弹性模型。这些模型将直接进行验证 通过细胞培养和机械实验。聚乙醇酸(PGA) 支架将种植软骨细胞，并在体外培养长达10年 几周。聚合物质量、细胞外基质成分质量(胶原蛋白和糖胺聚糖)、 构造的弹性模数将以规则的间隔被量化。 结果将被用于预测随时间变化的性能 细胞-聚合物结构，并深入了解支架的机制 降解和ECM合成。

DESCRIPTION: (provided by applicant) Tissue engineering is an emerging field in reconstructive surgery of dental and other craniofacial features consisting of skin, bone, or cartilage. In one common approach to tissue repair, cells capable of proliferating and synthesizing extracellular matrix (ECM) proteins are seeded on three-dimensional biodegradable polymer scaffolds. The scaffolds provide mechanical stability and geometric form to the construct until the cellular and biochemical components develop into competent tissue. In the design of engineered tissues, balancing the processes of biomaterial degeneration with tissue synthesis is critical in the initial phases of healing and long-term success. The combination of these two processes provides the mass and volumetric balance of materials that are intended to maintain the biological and mechanical health of repairing tissue. These complementary, time-dependent processes are often defined only empirically due to the lack of established parametric relationships. It is hypothesized that mathematical models can be developed to describe the dynamic mass state of concomitant scaffold degeneration and tissue synthesis. The model can be experimentally validated to confirm constituent quality and predict functional elastic parameters of the engineered tissue composite. The model will also provide design criteria and performance specifications to optimize the development of engineered tissues within the in vivo biologic and mechanical environment. It is intended that the general relationships defined in this project can be modified for all engineered tissue types. In the following proposal, two dynamic mass models (polyrmer and ECM mass) will be developed to define a construct elastic model of cartilage. These models will be directly validated through cell-culture and mechanical experiments. Poly-glycolic acid (PGA) scaffolds will be seeded with chondrocytes and cultured in vitro for up to 10 weeks. The polymer mass, ECM component masses (collagen and glycosaminoglycan), and elastic modulus of the constructs will be quantified at regular intervals. The results will be used to predict the time-dependent performance of cell-polymer constructs, and to gain insight into the mechanisms of scaffold degradation and ECM synthesis.