The molecular and biochemical mechanisms underlying Anthracnose resistance in Capsicum Acronym: ANTHRACAP

背景。炭疽病（Collectrichum spp）引起收获前和收获后的果实腐烂，导致全球经济损失。就甜椒而言，炭疽病感染已成为生产的主要障碍之一。我们的工业合作伙伴先正达（Syngenta）已经在自然多样性集合中发现了几种对炭疽病具有抗性的基因型。建立了对炭疽病菌抗性鉴定和鉴定的分离种群。这些产生的QTL现在已经固定，使研究基础的生化和分子机制负责炭疽病抗性性状。目的和目标。本项目旨在阐明辣椒（Capsicum）抗炭疽病的生化和分子机制。为实现这一目标，将执行以下目标。目的1。利用代谢组学、蛋白质组学和转录组学来确定对炭疽菌和潜在的其他真菌病原体具有抗性的潜在分子和生化成分。目标2。鉴定由辣椒基因型合成的具有潜在广泛应用前景的抗真菌代谢产物。这种方法将涉及天然产物化学和生物分析。目标3。在辣椒中使用病毒诱导基因沉默（VIGS）（由以前的iCASE学生开发）或通过在番茄中使用稳定转化来对(i)中确定的候选基因进行功能表征。

Background. Anthracnose (Collectrichum spp) causes pre and post-harvest fruit rot, leading to serve global economic losses. In the case of Pepper (hot and Sweet) anthracnose infections have become one of the main barriers to production. Our industrial partner, Syngenta, has identified several genotypes within collections of natural diversity that display resistance to Anthracnose. Segregating populations have been generated in which resistance to Colletotrichum species has been identified and mapped. These resulting QTL have now been fixed, enabling the study of underpinning biochemical and molecular mechanisms responsible for the Anthracnose resistance traits. Aims and Objectives. The proposed project activities aim to elucidate the biochemical and molecular mechanisms associated with Anthracnose resistance in pepper (Capsicum). To achieve this aim the following objectives will be carried out. Objective 1. The exploitation of metabolomics, proteomics and transcriptomics to ascertain the underlying molecular and biochemical components responsible for conferring resistance to Colletotrichum and potentially other fungal pathogens.Objective 2. Identify constitutive or induced antifungal metabolites synthesised by pepper genotypes that have potential broad applications as antimicrobial agents. This approach will involve natural product chemistry and bioassays.Objective 3. Functionally characterise candidate gene(s) identified in (i) using Virus Induced Gene Silencing (VIGS) in pepper (developed by previous iCASE students) or through the use of stable transformation in tomato.