Lens Inhibitor Proteins and Cataractogenesis

描述（由申请人提供）：30多年前，最初的 与年龄性白内障形成相关的透镜生物化学变化， 介绍了然而，在过去的十年里，很少有关于 由于缺乏人类白内障的研究，这个过程已经被收集起来。一 老年人透镜的共同特性是黄色发色团的积累， 它们与蛋白质结合。这些修改可以吸收UVA光和铅 与体外活性氧的形成有关这很重要因为 在原位照射在人的透镜上的UVA光的量是一千 紫外线强度是紫外线强度的10倍，所有紫外线都被透镜吸收。的 负责这种变黄的发色团将从老年人中分离出来。 晶状体和白内障及其光化学研究。因为看起来 人类的透镜中几乎没有氧气， 光化学将涉及活化的自由基的反应性 由UVA光在氩饱和溶液中形成。这些影响可能包括 抗氧化剂损失，保护透镜的酶失活， 蛋白质交联 这项补助金虽然只涉及UVA对透镜组织的影响，但仍有 24年前的名字

DESCRIPTION (provided by applicant): More than 30 years ago, the initial changes in lens biochemistry associated with age-onset cataract formation were described. In the past decade, however, little additional data pertaining to this process has been gathered due to a lack of human cataracts to study. A common property of aged human lens is the accumulation of yellow chromophores, which are bound to proteins. These modifications can absorb UVA light and lead to the formation of reactive oxygen species in vitro. This is important because the amount of UVA light impingement on the human lens in situ is a thousand times greater than UVB, and all of it is absorbed within the lens. The chromophores responsible for this yellowing will be isolated from aged human lenses and cataracts and their photochemistry studied. Since it seems likely that there is little or no oxygen in the human lens, much of this photochemistry will involve the reactivity of the activated free radicals formed by UVA light in argon-saturated solutions. These effects may include loss of antioxidants, inactivation of enzymes which protect the lens and protein crosslinking. This grant, while dealing solely with UVA affects on lens tissue, still carries the name from 24 years ago.