PROTEIN/RNA INTERACTIONS IN SPLICE SITE SELECTION

大多数真核基因都被内含子打断。初级阶段 必须对转录进行处理，以去除内含子并将 将编码序列编码成一个连续的mRNA分子。差异信使核糖核酸 分子可以从相同的前信使核糖核酸通过交替方式产生 当前信使核糖核酸含有多个内含子时的剪接。一个前提条件是 准确地从核前-mrna中剔除内含子是特异的。 识别和配对正确的5‘和3’位点。蜂窝 执行这一严格监管过程的机械涉及到一种有序的 组件的组装，包括异质核 核糖核蛋白、小核核糖核粒和其他 蛋白质因子，称为剪接因子。这个大型建筑群是已知的 作为剪接体。蛋白质-RNA相互作用是一种重要的机制 在剪接体组装和调节选择性剪接中， 已知在控制基因表达方面起着重要作用 发展就是差异化.我们将研究...的结构基础 剪接因子和hnRNP的序列特异性蛋白质-RNA相互作用 构成性和选择性前mRNA剪接的蛋白质。我们的 研究将有助于理解这些剪接体是如何 组件在调节前-mRNA剪接中实现其功能 原子水平。

Most eukaryotic genes are interrupted by introns. The primary transcript must be processed to remove the introns and to join the coding sequence into a contiguous mRNA molecule. Different mRNA molecules can be generated from the same pre-mRNA by alternative splicing when the pre-mRNA contains multiple introns. A prerequisite for the accurate excision of introns from nuclear pre-mRNA is specific recognition and pairing of the correct 5' and 3' sites. Cellular machinery carrying out this highly regulated process involves an ordered assembly of components, including heterogeneous nuclear ribonucleoproteins, small nuclear ribonuclear particles and additional protein factors, known as splicing factors. This large complex is known as the spliceosome. Protein-RNA interaction is an essential mechanism in spliceosome assembly and in regulating alternative splicing, which is known to play an important role in controlling gene expressing during development an differentiation. We will study the structural basis of sequence-specific protein-RNA interactions by splicing factors and hnRNP proteins in both constitutive and alternative pre-mRNA splicing. Our study will lead to the understanding of how these spliceosomal components achieve their function in regulating pre-mRNA splicing at an atomic level.