Intra-Oral Biofilm Formation

描述(申请人提供)：影响口腔的主要问题是生活在生物膜中的微生物在硬组织和软组织中定居。虽然人们对成熟的牙菌斑已经了解很多，但对牙菌斑形成过程中微生物种类的变化序列却知之甚少。对软组织微生物区系以及软组织微生物区系和硬组织微生物区系在生物膜发育过程中的相互作用更是知之甚少。两种重要的体液，唾液和GCF，沐浴在生物膜表面，在生物膜的发展中发挥着未知的作用。因此，这项应用的目的是确定在生物膜再发育早期发生的微生物种类的变化，并评估可能影响生物膜再发育的因素，包括唾液流量减少、缺乏自然牙列和牙龈炎症状态的变化。 SAL将比较60名牙周健康、60名牙周炎和60名戴假牙的无牙受试者从最初的牙科清洁到7天期间硬组织和软组织上正在形成的生物膜的微生物组成的变化。在基线时，分别从每颗牙齿的近中面采集牙菌斑样本，并从义齿上采集牙菌斑样本。唾液和八个口腔软组织表面的样本也将被收集。样本将通过棋盘DNA-DNA杂交对40种细菌的含量进行单独评估。在初步监测后，每组的所有牙齿受试者都将接受牙科清洁，同时义齿将进行清洁和消毒。每组随机抽取30名受试者进行全口软组织清洁和消毒。受试者将在7天内不进行口腔卫生，在此期间，将在第1、2、4和7天从随机选择的象限中采集牙菌斑和牙菌斑样本。牙菌斑样本将与唾液和软组织样本在同一地点采集。SA2将比较60名Sj？gren受试者和60名匹配对照组的牙菌斑和龈下菌斑、口腔软组织和唾液的微生物组成。将评估全口龋齿和牙周状况，并在基线时从每颗牙齿的近中方向采集牙面上和牙龈下菌斑的样本。此外，还将采集8个软组织样本和1个唾液样本。所有样品都将进行如上所述的微生物评估。所有受试者都将接受完整的口腔SAP，并将在28天后重新进行微生物学评估。拟议的研究应指出在生物膜形成过程中硬组织和软组织上发生的微生物变化的性质，以及唾液、牙周膜和自然牙列对生物膜形成的影响。

DESCRIPTION (provided by applicant): The major problems that affect the oral cavity are caused by organisms that live in biofilms colonizing the hard and soft tissues. While much has been learned about mature Dental plaque, little is known about the sequence of changes in microbial species that occurs during supra or subgingival plaque development. Even less is know about the soft tissue microbiota and the interplay between soft tissue microbiota and hard tissue microbiota during biofilm development. Two important bulk fluids, saliva and GCF, bathe the biofilm surfaces and play and undetermined role in biofilm development. Thus, the objectives of this application are to define the changes in microbial species that occur during early biofilm re-development and to evaluate factors that might influence this development including diminished salivary flow, lack of natural dentition and changes in gingival inflammatory status. SAl will compare the changes in microbial composition of developing biofilms on hard and soft tissues in 60 periodontally healthy, 60 periodontitis, and 60 denture-wearing, edentulous subjects from initial Dental cleaning to 7 days. Supra and subgingival plaque samples will be taken separately from the mesial aspect of each tooth at baseline and supragingival samples from dentures. Saliva and samples from eight oral soft tissue surfaces will also be collected. Samples will be evaluated individually for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. After initial monitoring, all dentate subjects in each group will receive a Dental cleaning while dentures will be cleaned and disinfected. Thirty randomly selected subjects in each group will receive full mouth "soft tissue cleaning and disinfection. Subjects will refrain from oral hygiene for 7 days during which time Supra and subgingival plaque samples will be taken from randomly selected quadrants at 1, 2, 4, and 7 days. Denture plaque samples will be taken at the same points as saliva and soft tissue samples.SA2 will compare the microbial composition of supra and subgingival plaque, oral soft tissues, and saliva in 60 Sj¿gren's subjects and 60 matched controls. Full-mouth caries and periodontal status will be assessed and samples of supra and subgingival plaque will be taken from the mesial aspect of each tooth at baseline. In addition, eight soft tissue samples and a saliva sample will be taken. All samples will be evaluated microbiologically as described above. All subjects will receive full mouth SAP and will be re-assessed microbiologically at 28 days. The proposed study should indicate the nature of the microbial changes that occur during biofilm development on hard and soft tissues and the influence that saliva, GCF and the natural dentition have on the developing biofilms.