CORE--MASS SPECTROMETRY LABORATORY

描述（改编自应用程序）：质量的功能 光谱核心是提供一个国家的最先进的实验室设施， 目的是加强稳定同位素在营养研究中的应用。这 研究将帮助我们了解营养吸收， 代谢和周转。具体来说，核心将涉及矿物 代谢（矿物质富集方法，包括建模）;测量 富集将用于检测生物转化的生物分子， 新陈代谢.总能量消耗的气体同位素定量测定 (TEE)用于基质氧化的气体同位素比测定。 质谱分析中心有着悠久的历史，在大学的 科罗拉多健康科学中心，并担任NIH研究资源 12年以上（至1991年）。通过强大的桥接 在医学院和儿科系的支持下，我们有 维持质谱分析设施及其专家工作人员。CNRU赠款 为这一核心提供了新的设备，并帮助稳定了工资 支持这一核心最重要的人力资源。 质谱核心实验室提供了进入一些国家的 最先进的质谱仪和训练有素的人员谁可以帮助每个 研究人员有效地规划和实施其具体研究项目。 该核心提供了一个仪器（VG 7070 H）和相应的硬件， 测量矿物质（铁、铜和锌）的富集;三种仪器 （MSD），专门用于测量简单的稳定同位素 生物分子（氨基酸、糖和脂肪）;仪器（HP 5989 A发动机） 用于特定目标化合物的开发分析和测量; 和VG Optima气体同位素比质谱仪，用于富集CO2和 2 H测定。然而，这种设备的高成本和 其维护的复杂性使得单个调查员不可能 独立于中心的功能。这显然是一个核心的例子， 从而扩大和增强核心成员的研究机会。

DESCRIPTION (adapted from the application): The function of the Mass Spectrometry Core is to provide a state-of-the-art laboratory facility for the purpose of enhancing the use of stable isotopes in nutrition research. This research will help us develop an understanding of nutrient absorption, metabolism, and turnover. Specifically, the core will be involved in mineral metabolism (mineral enrichment methodologies including modeling); measuring enrichments in biomolecules that will be sued to detect bioconversion and metabolism; gas isotope ration determinations for total energy expenditure (TEE); gas isotope ratio determinations for substrate oxidation. The Mass Spectrometry center has a long standing history at the University of Colorado Health Sciences Center, and served as an NIH Research resource facility for more than 12 years (until 1991). Through the strong bridging support of the School of Medicine and the Department of Pediatrics, we have maintained the Mass Spectrometry facility and its expert staff. The CNRU grant has provided new equipment for this core and has helped to stabilize the salary support for the most important human resources of this core. The Mass Spectrometry Core laboratory provides access to a number of state of the art mass spectrometers and trained personnel who can assist each investigator to effectively plan and implement their specific research project. This core provides an instrument (VG 7070H) and corresponding hardware for the measurement of enrichment of minerals (Fe, Cu, and ZN); three instruments (MSDs) that are dedicated to the measurements of stable isotopes in simple biomolecules (amino acids, sugars, and fats); an instrument (HP 5989A Engine) is used for developmental assays and measurement of specific target compounds; and a VG Optima gas isotope ratio mass spectrometer for both enriched CO2 and 2H determinations. However, the high expense of this equipment and the complexity of its maintenance make it impossible for an single investigator to function independent of the center. Thus, this is clearly an example of a core which extends and enhances the research opportunities of the core members.