MOLECULAR BIOPHARMACOLOGY LAB & TISSUE CULTURE LAB CORE

描述：应用程序请求资金以继续开发和 加强MBL和TCL，以提高支助服务水平 对调查人员来说，并符合在 生物技术。这些设施将有能力：1)隔离和 在体外细胞培养中保持特定组织的不同细胞类型 系统；2)分离和鉴定生物分子，如蛋白质、酶、 和核酸；3)产生单抗和多克隆抗体；4) 生产各种用途的重组蛋白；5)分离感兴趣的基因 以及它们在基因治疗中的潜在用途；以及6)进行体外检测 细胞和非细胞化合物的细胞毒性和生物活性。这个 MBL和TCL设施的主要总体目标是：1)提供研究 基础设施，促进教师和研究生的研究培训 学生；2)创造促进信息交流的研究环境 和技术；3)增强研究能力和竞争力 研究调查人员获得外部资金；以及4)制度化 MBL和TCL核心设施。

Description: The application requests funds to continue the development and enhancement of the MBL and the TCL, to increase the level of support services to the investigators, and to conform with the technological advances made in biotechnology. These facilities will have the capability to: 1) isolate and maintain different cell types from specific tissue in in vitro cell culture systems; 2) isolate and characterize biomolecules such as proteins, enzymes, and nucleic acids; 3) generate monoclonal and polyclonal antibodies; 4) produce recombinant proteins for various uses; 5) isolate genes of interest and their potential use in gene therapy; and 6) perform in vitro assays for cytotoxicity and bioactivity of cellular and non-cellular compounds. The major general goals of the MBL and TCL facilities are to: 1) provide research infrastructure to facilitate research training of both faculty and graduate students; 2) create a research environment that leads to information exchange and technology; 3) enhance the research capabilities and competitiveness of research investigators to obtain extramural funding; and 4) institutionalize the MBL and TCL core facilities.