Temperature and vernalisation regulation of seed properties in Brassica napus

在拟南芥中，春化和结实期的温度在决定种子大小和活力等种子特性方面都起着重要作用。在作物中，这些效应可能对作物产量和田间种子质量有重要影响，但在芸苔属作物中尚未深入探索。2018年秋季，来自sLOLA芸苔菜籽和蔬菜优化（BRAVO）的团队进行了一项大型实验，在油菜多样性小组中对多种表型性状进行评分。实验包括在三种温度下完成春化的复制植物，在两种不同的持续时间内，然后将每种处理的植物在两个不同的温度下的两个不同的温室中播种。该试验计划对植物的开花时间和荚果破碎进行表型分析，但没有计划对结果种子进行表型分析。该项目的第一个目标是开始分析植物产生的种子，以确定春化温度、春化持续时间和种子定型温度对油菜种子品质的影响。第二个目标是利用MapMan软件管道分析来自种子的现有转录组数据，以确定与受温度影响的种子大小、含油量和活力相关的途径。

In Arabidopsis vernalisation and temperature during seed set both play an important role in determining seed properties such as seed size and seed vigour. In crops these effects can be important for crop yield and seed quality in the field, but have yet to be explored in depth in Brassica crops.In autumn 2018 the team from the sLOLA Brassica Rapeseed and Vegetable Optimisation (BRAVO) sowed a large experiment to score multiple phenotypic traits in an Oilseed Rape diversity panel. The experiment consists of replicate plants completing vernalisation at three temperatures for two different durations, and then with plants from each treatment setting seeds in two different glasshouses maintained at two temperatures. The experiment has been set up with plans for phenotyping the flowering time and pod shattering of the plants but no plans for phenotyping the resulting seeds.The first aim of this project is to begin to analyse the seeds produced by the plants to define the effect of vernalisation temperature, duration of vernalisation and seed set temperature on oilseed rape seed qualities. The second aim is to analyse existing transcriptome data from seeds to identify pathways associated with seed size, oil content and vigour that are affected by temperature, using MapMan software pipeline.