Multimodal Comparative Study of Matched Human iPSC and Biopsy-Derived Muscle Progenitor Cells for Regenerative Therapies

骨骼肌是人体内一种丰富的组织，可因创伤或一系列遗传、代谢和神经肌肉疾病而功能受损。功能性人骨骼肌祖细胞(成肌细胞)可以从人诱导的多能干细胞(HiPSC)中获得。该项目将建立一个结合形态、细胞标记染色和细胞信号通路激活的高含量细胞表型平台，以比较HiPSC来源的成肌细胞和供体骨骼肌成肌细胞的表型。该项目将产生有关使用HiPSC来源的成肌细胞进行疾病建模、测试新疗法和开发再生药物的有价值的新信息。

Skeletal muscle is an abundant tissue in the human body that can be functionally compromised due to trauma or a range of genetic, metabolic and neuromuscular diseases. Functional human skeletal muscle progenitor cells (myoblasts) can be derived from human induced pluripotent stem cells (hiPSC). This project will establish a high-content cell phenotyping platform combining morphological, cell marker staining and activation of cell signaling pathways to compare the phenotype of hiPSC-derived myoblasts with myoblasts isolated from donor skeletal muscle. The project will generate valuable new information regarding use of hiPSC-derived myoblasts for disease modelling, testing new therapeutics and developing regenerative medicines.