Tissue Homogenizaton by Pressure Cycling Technology

描述(申请人提供)：使用加压循环的应用程序 正在开发一种技术(PCT)来裂解细胞并使组织匀浆 自动化的生物样本处理。这项技术适用于 低吞吐量和高吞吐量应用中的自动化流程。我们的第一阶段 研究表明，PCT对动物和动物的均质都非常有效 植物组织样本。PCT释放的核酸与 常规方法，适用于聚合酶链式反应和逆转录聚合酶链式反应分析。细菌 样品可以在简单的裂解液中裂解，粗裂解产物由 PCT适合于PCR扩增。在这项第二阶段研究中，组织 同质化将用各种动物和植物样本进行评估。 有不同程度的困难。PCT组织裂解剂的配伍研究 程序和一般的核酸提取方法将被评估。 将开发多个并行样品处理。的最终目标是 该项目旨在展示PCT裂解物在各种 使用核酸和蛋白质的生物分析。迈向 这一同质化进程的商业化进程将继续进行 完成这项第二阶段研究。 建议的商业应用： 用于样品制备的压力循环技术(PCT)的发展最终将允许生物样品的自动化均质和裂解，例如细菌、动物和植物组织。PCT裂解物可用于在自动化过程中需要均质生物样品的各种应用中。PCT均质技术的成功开发将有利于基于分子的分析方法在基因组学、医学、法医学、农业、制药、兽医和临床应用中的应用

DESCRIPTION (provided by applicant): An application using pressure cycling technology (PCT) is being developed to lyse cells and homogenize tissues for automated biological sample processing. This technology is feasible for automated processes in both low and high throughput applications. Our Phase I study demonstrated that PCT is very powerful to homogenize both animal and plant tissue samples. Nucleic acids released by PCT were comparable to conventional methods, and suitable for PCR and RT-PCR analysis. Bacteria samples could be lysed in simple lysis solution and crude lysate generated by PCT was suitable for PCR amplification. In this phase II study, tissue homogenization will be evaluated with a variety of animal and plant samples with different degrees of difficulties. Compatibility of the PCT tissue lysis procedure and the general nucleic acid extraction methods will be evaluated. Multiple parallel sample processing will be developed. The final objective of the project is to demonstrate utilities of PCT lysate in a variety of biological assays using nucleic acids and proteins. Steps towards commercialization of this homogenization process will be pursued upon completion of this Phase II study. PROPOSED COMMERCIAL APPLICATOIN: The development of the pressure cycling technology (PCT) for sample preparation will ultimately permit automated homogenization and lysis of biological samples, such as bacteria, animal and plant tissues. The PCT lysate may be utilized in a variety of applications that require homogenized biological samples in automated processes. The successful development of the PCT homogenization technology will benefit molecular based analytical procedures in genomics, medical, forensic, agricultural, pharmaceutical, veterinary and clinical applications