A High Yield Affinity Cell Separation Technology

描述（由申请人提供）该项目的长期目标是开发一种高产量、基于亲和的细胞分离技术。这项技术有几个有价值的潜在临床应用。我们将重点从血液中分离罕见的循环肿瘤细胞。这项技术将促进可重复试验的发展，以便在转移发生之前和手术可以治愈的早期时间点检测隐匿性癌症。将细胞捕获到带有抗体的固体表面的限制因素是细胞与其被束缚的表面之间的附着位点的数量。增加细胞和固相之间的连接数量有望增加附着的强度和最终的细胞产量。我们的技术开发计划侧重于一种以对数方式增加附件链接数量的方法。在第一阶段的努力中，我们将开发两种新型配体，它们在固体表面上的附着密度极高。我们将这些配体与淋巴细胞特异性抗体结合并测试其有效性。具体来说，我们将测量这些配体介导固体表面强大附着的能力，并将它们与传统的、现有的亲和细胞分离技术进行比较

DESCRIPTION (provided by applicant) The long-term objective of this project is the development of an exceptionally high yield, affinity-based cell separation technology. The technology has several valuable potential clinical applications. We will focus on the isolation of rare circulating neoplastic cells from blood. This technology will facilitate the development of a reproducible test for the detection of occult cancers at an early point in time, before metastases occur and when surgery could be curative.The limiting factor in capturing cells to a solid surface with antibodies is the number of attachment sites between a cell and the surface to which it is to be tethered. Increasing the number of links between the cell and the solid phase will be expected to increase the strength of attachment and the resulting cell yield. Our technology development program focuses on a method to logarithmically increase the number of attachment links. In this Phase I effort, we will develop two novel ligands that demonstrate extremely high densities of attachment to solid surfaces. We will conjugate these ligands to lymphocyte-specific antibodies and test their effectiveness. Specifically, we will measure the ability of these ligands to mediate a powerful attachment to the solid surface and compare them to conventional, existing affinity cell separation technologies