CORE--TRANSGENIC MOUSE FACILITY

基因工程小鼠的使用呈指数级增长。转基因缺陷小鼠目前被广泛用于研究：恶性转化过程、抑癌基因、化学预防、致癌和器官特异性癌症。事实上，小鼠的基因操作几乎被用于生物医学研究的每个领域。它可以说是最有力的工具，可用于解剖基因功能的生理学和病理生理学。然而，由于在各个实验室内建立这些程序所需的费用高昂，技术复杂，因此必须支持这些技术，将其作为一种共享资源。这就需要一个有效的和具有成本效益的共同设施，使个别研究人员既有转基因小鼠表达的目的基因和小鼠携带的基因破坏为他们创造。本资源提供的技术将这些强大的分析工具交给我们所有的研究人员。当与该中心的组织学和分子病理学设施提供的其他现代技术结合使用时，它为我们的研究人员提供了无限的机会，可以同时从多个角度研究发病过程。我们目前的设施有一个既定的跟踪记录，使创建两个转基因小鼠和敲除小鼠品系的多个癌症中心实验室。如果没有这样一个集中的资源，大多数实验室将无法利用这些强大的模型，严重限制了我们的生物医学和癌症研究界的研究和授予机会。UCSD癌症中心的转基因小鼠共享资源在社区中受到广泛尊重，并被视为现代癌症研究的重要资源。它大大提高了研究的质量、数量和创造力，同时也提高了成本效益和效率。

The use of genetically engineered mice has grown exponentially. Transgenic deficient mice are now widely used to study: the process of malignant transformation, tumor suppressor genes, chemoprevention, carcinogenesis and organ-specific cancers. In fact, the genetic manipulation of mice is used in virtually every field of biomedical investigation. It is arguably the most potent tool available to dissect gene function in physiology and pathophysiology. However, because of the prohibitive costs and the technical sophistication required to establish these procedures within individual laboratories, it is essential to support these technologies as a shared resource. This requires an efficient and cost-effective common facility that enables individual investigators to have both transgenic mice expressing genes of interest and mice carrying gene disruptions created for them. The techniques available from this Resource place these potent tools for analysis in the hands of all of our investigators. When used in combination with the other modern techniques supplied by the Center's Histology and Molecular Pathology facilities, it gives our investigators unlimited opportunities to examine pathogenetic processes from several perspectives simultaneously. Our current facility has an established track record of enabling the creation of both transgenic mice and knockout mouse strains for multiple Cancer Center laboratories. Without such a centralized resource, most laboratories would not be in a position to utilize these pow4erful models, severely limiting the research and granting opportunities for our biomedical and cancer research community, severely limiting the research and granting opportunities for our biomedical and cancer research community. The Transgenic Mouse Shared Resource of UCSD Cancer Center is widely respected in the community and regarded as an essential resource for modern cancer research. It substantially improves the quality, quantity and creativity of the research, while also enhancing cost-effectiveness and efficiency.