CORE--BIOSENSOR/INTERACTION ANALYSIS FACILITY

生物传感器/相互作用分析核心于1997年正式成立，以满足日益增长的对大分子相互作用分析的需求。最初的资金部分由癌症中心通过核心赠款发展基金和非核心赠款来源以及医学院提供。欧文·柴肯博士，医学教授，担任设施主任。柴肯博士是使用生物传感器进行动力学相互作用分析以及生物分子识别领域的国家专家。柴肯博士的实验室是首批将生物传感技术应用于特定大分子相互作用系统的实验室之一。这项生物传感器工作源于柴肯博士早期的工作，即利用固定化配体开发用于相互作用分析的分析亲和层析。柴肯博士计划并建立了生物传感器/相互作用分析矿石，现在担任该中心的设施主任。该设施的技术总监。Gabriela Canziani博士在酶动力学、酶催化部位的结构-功能以及分光光度方法方面拥有十多年的经验，包括荧光光谱和脂蛋白相互作用的生物物理分析。在这一启动期间，使用量稳步增长。绝大多数用户是回头客，他们通过扩大当前项目的范围和启动新项目来增加他们的使用量。在最近的预算年度，83%的使用是由癌症中心成员完成的，79%是由癌症中心成员通过同行评审资助完成的。随着这一核心成为NCI批准的癌症中心共享资源，以及随着更多的研究人员熟悉其日益增长的能力，癌症中心成员在下一个项目期的使用量将会增加。

The Biosensor/Interaction Analysis Core was formally established in 1997 to meet the growing need for macromolecular interaction analysis. Initial funding was partially provided by the Cancer Center, through both Core Grant Developmental Funds and non-Core Grant sources, as well as by the School of Medicine. Irwin Chaiken, PhD, Professor of Medicine, serves as Facility Director. Dr. Chaiken is a national expert in kinetic interaction analysis using biosensors as well as in the field of biomolecular recognition. Dr. Chaiken's laboratory was one of the first to apply the use of biosensory technology to specific macromolecular interaction systems. This biosensor work emerged from Dr. Chaiken's earlier work in the development of analytical affinity chromatography for interaction analysis using immobilized ligands. Dr, Chaiken planned and established the Biosensor/Interaction Analysis ore and now serves as it's Facility Director. The facility's Technical Director,. Gabriela Canziani, PhD, has more than ten years of experience in enzyme kinetics, structure-function of the catalytic sites of enzymes, and spectrophotometric methods, including fluorescence spectroscopy and biophysical analysis of lipid- protein interactions. Usage has increased steadily during this start-up period. The vast majority of users are repeat customers who are increasing their usage by both expanding the scope of current projects and initiating new projects. During the most recent budget year, 83% of usage was done by Cancer Center members and 79% was by Cancer Center members with peer- reviewed funding. Increased usage by Cancer Center members is very much anticipated for the next project period as this core becomes an NCI- approved Cancer Center Shared Resources and as more investigators become familiar with its growing capabilities.