CORE--MOLECULAR METHODS

描述：(申请者？S描述)共享的VCC分子方法 资源将成为新的共享资源。目标是将工作集中到 发展和推广细胞和分子技术方面的专业知识，从而 增强VCC调查人员的研究能力。一个主要目标将是 探索和充分利用几种新仪器的功能 可供VCC调查人员使用，因为它们与方案相关 研究，并帮助设计和实施新的方法，以实现当前 研究目标。在规划这一核心时，VCC调查人员确定了优先事项 需要技术开发的领域，以绕过当前 实验设计和/或数据收集中的限速步骤。这些 包括：1.异位基因表达；2.基因定量分析 在动物和人中表达的蛋白质和基因的分析 组织样本。这个核心的主要功能将是方法和化验 发展。目标是整合开发和开发所需的资源 用于分析基因变化水平和后果的扩展技术 表情。通过这样做，这个新的核心设施可以显著增强 VCC调查人员的研究潜力。

Description: (Applicant?s Description) The VCC Molecular Methods Shared Resource will be a new shared resource. The goal is to centralize efforts to develop and extend expertise in cell and molecular techniques and thereby enhance the research power of VCC investigators. A major goal will be to explore and take full advantage of the capabilities of several new instruments available to VCC investigators as they are relevant to the programmatic research, and to help design and implement new approaches toward current research goals. In planning this core, VCC investigators identified priority areas for techniques development that are needed to circumvent the current rate-limiting steps in experimental design and/or data collection. These include: 1. ectopic gene expression, 2. quantitative analysis of gene expression and 3. analysis of protein and gene expression in animal and human tissue samples. A major function of this core will be in methods and assay development. The goal is to consolidate resources needed to develop and extend techniques for analyzing levels and consequences of changes in gene expression. In doing so, this new core facility can significantly enhance the research potential of VCC investigators.