CORE--FLOW CYTOMETRY AND FLUORESCENCE IMAGING FACILITY

描述：(申请人描述)流式细胞术/荧光法 影像共享资源为癌症中心研究人员提供先进的 用于细胞研究的仪器。为此，它的目标是提供 最先进的流式细胞仪和图像分析系统用于采集 数据；提供用于细胞分析的计算机硬件和软件 从这些仪器获得的数据；培训科学家使用 仪器和软件；开发和实施细胞的新技术 分析；并鼓励科学家之间就共同的方法进行交流 利息。 对于流式细胞仪，共享资源拥有两台Becton Dickinson仪器： 升级为TurboSort的FACStar Plus VIS/UV七参数细胞仪 分选和高级应用以及FACScan五参数细胞仪 常规分析。资源实验室也有三个图像分析 系统：Bio-Rad MRC-1024氪/氩激光共聚焦系统；子午线 Ultima UV/VIS Ar激光共聚焦系统和Zeiss Axiophote 带有冷却的电荷耦合器件探测器的显微镜。有，在共享的 资源，一个Silicon Graphics工作站，三台联网的Macintosh 工作站，以及用于出版物质量的Codonics染料升华打印机 彩色打印。 在96-97财年，流式细胞仪/荧光成像共享资源是 由来自34个实验室的大约100个不同的人使用 调查人员。在此期间，在总共2,858个计费小时中，有2,741个 由癌症中心成员使用(占总账单使用的95.9%)。在 目前，癌症中心研究人员使用共享资源暂存 现役赠款每年的直接费用总额为10,399,757美元。在这笔钱中，97美元 %来自同行审查的拨款(84.2%来自NIH)。

Description: (Applicant's Description) The Flow Cytometry/Fluorescence Imaging Shared Resource provides Cancer Center investigators with advanced instrumentation for the study of cells. To this end, it aims to provide state-of-the-art flow cytometers and image analysis systems for acquisition of data; to provide computer hardware and software for the analysis of cellular data derived from these instruments; to train scientists in use of the instruments and software; to develop and implement new techniques for cell analysis; and to encourage communication among scientists on methods of common interest. For flow cytometry, the Shared Resource has two Becton Dickinson instruments: a FACStar Plus VIS/UV seven-parameter cytometer with TurboSort upgrade for sorting and advanced applications and a FACScan five-parameter cytometer for routine analysis. The Resource laboratory also has three image analysis systems: a Bio-Rad MRC-1024 krypton/argon laser confocal system; a Meridian Ultima UV/VIS argon laser confocal system; and a Zeiss Axiophot photomicroscope with a cooled CCD detector. There are, within the Shared Resource, a Silicon Graphics workstation, three networked Macintosh workstations, and a Codonics dye-sublimation printer for publication-quality color printing. During FY 96-97, the Flow Cytometry/Fluorescence Imaging Shared Resource was used by approximately 100 different individuals from the labs of 34 investigators. For that period, 2,741 out of a total of 2,858 billed hours of use were by Cancer Center members (95.9 percent of total billed use). At the present time, Cancer Center investigators using the Shared Resource hold active grants totaling $10,399,757 in annual direct costs. Of this sum, 97 percent is from peer-reviewed grants (84.2 percent from NIH).