Profiling Cytochrome P450 Enzymes Using Chemical Probes

细胞色素P450是一个超家族的酶，负责生命树的I期新陈代谢和生物合成。在植物中对CYP450的研究还不够深入，对关键因素的更多了解将有助于更知情地设计植物保护产品(除草剂、化肥、杀虫剂)。本文概述的项目旨在开发用于研究这些酶的探针库。将使用传统的化学合成方法，并在常规生物评估之后，在整个项目中设计和合成新一代探测器。BBSRC与该项目相关的优先领域：食品、营养和可持续地提高农业生产全球粮食安全

CYP450s are a superfamily of enzymes responsible for phase I metabolism and biosynthesis across the tree of life. CYP450s are understudied in plants and a greater knowledge of the key players would allow for more informed design of plant protection products (herbicides, fertilisers, insecticides). The project outlined herein aims to develop a probe library for use in the studying of these enzymes. Traditional chemical synthesis methods will be employed and, following routine biological evaluation, next generation probes will be designed and synthesised throughout the project.BBSRC priority areas related to the project:Food, nutrition and healthSustainably enhancing agricultural productionGlobal food securityAfS, ENWW