CORE--GENOMICS

描述：（改编自申请人的叙述）基因组学核心由两个部分组成，一个在麻省理工学院，由兰德博士指导，另一个在威斯康星医学院，由雅各布博士指导。该核心生成所有与单核苷酸多态性（snp）相关的湿实验室基因组数据，测序，使用简单序列长度多态性（SSLPs）对大鼠进行基因分型，SSLPs的生成以及大鼠和人类的辐射杂交图谱（如果需要）。该核心支持所有四个项目。Drs。Jacob和Lander都是各自机构遗传/基因组中心的主任。这些中心有必要的机器人、PCR机、ABI测序仪和LJL Analyst板阅读器来完成本核心的任务。该核心的Whitehead组件将负责所有SNP工作，包括检测和基因分型。对于项目1，基因组学核心将测试500个非裔美国人病例和500个非裔美国人对照的snp，这些病例或与项目2中的血压或相关表型有关，或与文献中的血压或相关表型有关，或组成合作者。对于项目2，Genomics Core将开发至少三个与血压或相关表型相关的人类基因组区域的snp（使用基因组序列）。基因组学核心的MCW组件将与项目3密切合作，对经济大鼠和基因大鼠进行基因分型。为了生成分辨率为0.5 cM和1 cM的同源线，我们需要开发额外的sslp。MCW组件也将在项目4中的比较映射中发挥作用。比较图谱的一个方面是为目标微阵列开发DNA探针（在侧面）。这将通过设计基于预测基因的PCR引物来完成（项目4），然后在辐射杂交图谱上测试这些预测基因，以确保它们在感兴趣的QTL区域内。

DESCRIPTION: (Adapted from the applicant's narrative) The Genomics Core consists of two component, one at MIT under the direction of Dr. Lander, and the other at the Medical College of Wisconsin component under the direction of Dr. Jacob. This core generates all wet lab genomic data related to single nucleotide polymorphisms (SNPs), sequencing, genotyping of rats using simple sequence length polymorphisms (SSLPs), generation of SSLPs, and radiation hybrid mapping in rats and human (if needed). This core supports all four projects. Drs. Jacob and Lander are both Directors of Genetic/Genomic Centers at their respective institutions. These centers have the necessary robotics, PCR machines, ABI sequencer, and LJL Analyst plate reader for completing the tasks in this core. The Whitehead Component of this core will be responsible for all SNP work, which includes detection and genotyping. For Project 1, the Genomics Core will test SNPs in 500 African American cases and 500 African American controls that have been either linked to blood pressure or a related phenotype in Project 2 or associated with blood pressure or related phenotype in the literature, or form a collaborator. For Project 2, the Genomics Core will develop SNPs (using genomic sequence) for at least three regions of the human genome linked to blood pressure or related phenotype. The MCW component of the Genomics Core will work closely with Project 3 to conduct the genotyping of the consomic rats and the congenic rats. To generate congenic lines to the resolution of 0.5 and 1 cM, we will need to develop additional SSLPs. The MCW component will also play a role in the comparative mapping within Project 4. One aspect of the comparative map will be to develop DNA probes (on the side) for the targeted microarray. This will be accomplished by designing PCR primers based on predicted genes (Project 4) and then testing these predicted genes on the radiation hybrid map to make sure they are within the QTL region of interest.