CORE--IMAGING

目的： 成像核心将具有两项服务功能。第一个功能是 为最先进的形态方法提供技术支持。这个 第二个功能是收集和评估尸检中的人体组织， 外科手术切除和移植程序。 1.为最先进的形态提供技术专业知识 方法：研究方法。这些方法包括常规方法和专门的定量方法 组织学和电子显微镜、免疫组织化学和原位观察 杂交。我们在活体实验方面有相当多的专业知识 动物研究、活体实验和人体组织分析。 2.从身体解剖、手术切除和 移植程序。成像核心的第二个功能是 利用阿尔伯廷博士建立的人类组织库， 齐默尔曼博士的无价协助。迈克尔和麦金太尔。是这样的 在ARDS中对人类肺部的研究迫切需要相互关联 支气管肺泡灌洗和血浆标本中各因子的测定 并将其作为免疫组织化学和其他研究的“黄金标准” 在急性肺损伤的动物模型中，如其他人所认识到的(Pittet et 等人，1997年)。我们对未来的扩建计划感到特别兴奋 超出特定项目的直接目标的分析，一旦 已经定义了原位内皮细胞的炎症表型， 分析信号因子、表面黏附分子、 以及炎症环境中其他细胞类型中的其他关键变量 急性呼吸窘迫综合征的肺脏。令人兴奋的未来计划之一是分享我们的人类 组织银行和其他SCOR项目，例如我们正在计划与Dr。 哈德森在西雅图的SCOR小组。 重要意义： 成像核心的贡献将大大改善我们的 对失调的细胞间相互作用和细胞间关系的理解 在ARDS的不同阶段发出信号(例如，早期损伤与晚期损伤， 以及损伤与修复之间的关系)。

PURPOSE: The Imaging Core will have 2 service functions. The first function is to provide technical support for state-of-the-art morphological methods. The second function is to collect and evaluate human tissue from autopsies, surgical resections and transplant procedures. 1. To provide technical expertise for state-of-the-art morphological methods. These methods include routine and specialized quantitative histology and electron microscopy, immunohistochemistry, and in situ hybridization. We have considerable expertise with in vivo experimental animal studies, in vivo experiments, and human tissue analyses. 2. To collect human tissues from autopsies, surgical resections and transplant procedures. The second function of the Imaging Core is to exploit the human tissue bank that Dr. Albertine established, with the invaluable assistance of Drs. Zimmerman. Michael and McIntyre. Such studies of the human lung in the ARDS are desperately needed to correlate with measurements of factors in bronchoalveolar lavage and plasma samples and to use as a "gold standard" for immunohistochemical and other studies in animal models of acute lung injury, as recognized by others (Pittet et al., 1997). We are particularly excited by the future plan of extending the analysis beyond the immediate goals of the specific projects, once the inflammatory phenotype of in situ endothelial cells has been defined, to analysis of the display of signaling factors, surface adhesion molecules, and other key variable sin other cell types in the milieu of the inflamed lung in ARDS. One of the exciting future plans is to share our human tissue bank with other SCOR programs, such as we are planning with Dr. Hudson's SCOR group in Seattle. SIGNIFICANCE: The contributions of the Imaging Core will substantially improve our understanding of dysregulated cell-cell interactions and intercellular signaling at different stages of the ARDS (e.g., early versus late injury, and injury versus repair).