CORE--TISSUE AND CELL LABORATORY

（根据申请人？组织与细胞核心实验室 将为急性肺损伤SCOR中的这些项目提供支持服务 需要功能研究的文件;这些研究将使用 在不同项目中获得的动物和临床材料。的 可提供的支持服务包括传统的光学显微镜、电子显微镜 显微镜、定量形态测定和分子生物学技术。的 定量形态测量技术可在光镜和显微镜下进行。 和超微结构水平提供定量相关性， 在其他方案中观察到的功能变化。分子生物学 技术包括基本克隆、质粒制备和原位 杂交方法 组织和细胞核心实验室？的主要互动将与项目 1、3和6。项目1将研究急性肺损伤患者， 肺动脉血栓内膜切除术项目3涉及肺的作用 急性肺损伤中表面活性物质系统及干预措施 有利于项目6关注PTHRP在急性肺损伤中的生物学。 通过肺活检从临床研究中的患者获得的组织 将由组织和细胞核心处理 实验室这些样本将由核心单元负责人审查 证实病理诊断。然后根据协议 将进行定量形态学研究，以确定 肺损伤，例如，急性损伤、纤维化、血管损伤和 间质和空气-血管间隙定量。质量评估 将报告存在的肺部炎症与各种 实验干预。适当时，将制备组织， 免疫组化 组织和细胞核心实验室的服务将继续进行 目前急性呼吸衰竭SCOR在UCSD的合作。

(Adapted from the Applicant?s Abstract) The Tissue and Cell Core Laboratory will provide support services to those projects in the Acute Lung Injury SCOR that require documentation of the functional studies; these studies will use both animal and clinical material obtained in the different Projects. The support services available include conventional light microscopy, electron microscopy, quantitative morphometry and molecular biology techniques. The quantitative morphometric techniques are available at both light microscopic and ultrastructural levels to provide quantitative correlation with the functional changes observed in other protocols. The molecular biology techniques include basic cloning, plasmid preparation and in situ hybridization. The Tissue and Cell Core Laboratory?s main interactions will be with Projects 1, 3, and 6. Project 1 will be studying patients with acute lung injury after pulmonary thromboendarterectomy. Project 3 addresses the role of the lung surfactant system in acute lung injury and what interventions may be beneficial. Project 6 focuses on the biology of PTHRP in acute lung injury. Tissues obtained from patients in the clinical studies either by lung biopsy during surgery or at autopsy will be processed by the Tissue and Cell Core Laboratory. These specimens will be reviewed by the Core Unit Leader confirming the pathologic diagnosis. Then, depending on the protocol, quantitative morphometric studies will be conducted to determine the extent of lung injury, e.g., acute damage, fibrosis, vascular injury and alterations in interstitial and air-vascular space rations. Qualitative assessments of the pulmonary inflammation present will be reported to correlate with the various experimental interventions. When appropriate, tissues will be prepared for immunohistochemistry. The services of the Tissue and Cell Core Laboratory will continue the ongoing collaborations of the present Acute Respiratory Failure SCOR at UCSD.