Core--RNA expression profiling

RNA表达谱核心。综合分析RNA表达在不同的细胞类型已经革命性的使用DNA微阵列分析。这种方法可以用少量的细胞或组织定量地评估数千种基因的表达模式。该分析旨在为在体外和体内分析中形成本提案基础的每个目标所研究的模型开发遗传标记。这些标记的识别可以用来促进这些模型假设的验证。使用RNA标记应该是一种确定细胞表达谱的可靠方法。一旦确定了一组标记，这些序列就可以通过其他方法（例如原位杂交）进一步测试假设。Core E将与项目1和2的研究人员合作，从培养细胞和实验操作的血管组织中识别标记物，这些标记物可以与从人类GA和对照动脉中制备的RNA进行比较。

RNA Expression Profiling Core. A comprehensive analysis of RNA expression in distinct cell types has been revolutionized by the use of DNA microarray analysis. This methodology can assess the expression patterns of thousands of genes in quantitative terms with small amounts of cells or tissue. This analysis seeks to develop genetic markers for the models under study in each aim in the in vitro and in vivo analyses that form the basis of this proposal. Identification of such markers can then be used to facilitate the validation of the hypotheses of these models. The use of RNA markers should be a reliable method for defining cellular expression profiles. Once a set of markers has been identified these sequences can then be used by additional methods (e.g., in situ hybridization) to further test the hypotheses. Core E will work with investigators in Projects 1 and 2 to identify markers from cultured cells and from experimentally manipulated vascular tissues that can be compared with RNA prepared from human GA and control arteries in Core D.