Core--Animal

本PPG的4个项目所需的小鼠将由动物中心繁殖和饲养。核心将为eNOS、nNOS、EC-SOD、NAD（P）H氧化酶-p47/phox亚基和D5-R 5个已开发菌株安排育种对的购买和转让。将从最初的育种者中培养出支持8-14周龄成年雄性小鼠的集落。将饲养杂合（+/-）小鼠，以在同一窝中提供纯合（+/+）野生型和纯合（-/-）缺失小鼠，以最大化对照组的遗传背景。核心已制定详细计划，规定了生成足够研究动物所需的小鼠数量。核心将维护与每只小鼠耳标识别号相关的相关信息数据库。核心将从断奶小鼠中采集血液用于基因分型，基因分型将在分子核心中进行。在放置皮下微型泵之前和期间测量每只动物的收缩压。微型泵将含有载体或血管紧张素II（Ang II）。将采集24小时尿液并分析8-异前列烷、脂质过氧化、一氧化氮代谢产物和肌酐。Core还将在微型泵放置之前和期间，通过无线电遥测直接测量每个单基因缺失菌株的子集中的有意识平均动脉血压。该中心将在与动物和分析有关的所有领域为每个项目提供技术支持。

Mice required for the 4 projects of this PPG will be bred and maintained by the Animal Core. The Core will arrange the purchase and transfer of breeder pairs for 5 developed strains: eNOS, nNOS, EC-SOD, NAD(P)H oxidase-p47/phox subunit, and D5-R. Colonies to support adult male mice of 8-14 weeks will be developed from the initial breeders. Heterozygous (+/-) mice will be bred to provide both homozygous (+/+) wild type and homozygous (-/-) deleted mice in the same litter, to maximize the genetic background of the control groups. The Core has in place a detailed plan that specifies the number of mice required to generated sufficient study animals. The Core will maintain a database of pertinent information that correlates to the ear tag identification number of each mouse. The Core will collect blood from weaning mice for genotyping, which will be performed fin the Molecular Core. The systolic blood pressure of each animal will be measured before and during placement of subcutaneous mini-pumps. Mini-pumps will contain either vehicle or angiotensin II (Ang II). 24 hour urine will be collected and analyzed for 8-isoprostane, lipid peroxidation, nitric oxide metabolites, and creatinine. The Core will also measure the conscious mean arterial blood pressure directly by radio telemetry in a subset of each single-gene deleted strain, before and during mini-pumps placement. The Core will provide technical support for each project in all areas relating to animals and analysis.