CORE--MOLECULAR BIOLOGY

（摘自申请人摘要）核心的总体目标是 进行诊断测试镰状细胞贫血和地中海贫血的DNA 水平，并为研究人员提供分子生物学支持， 中心核心将执行镰状细胞贫血，诊断，阿尔法- 地中海贫血（alphaSEA、alphaMED、alpha20.5）、HPFH-1、HPFH-2和 使用聚合酶链反应确定单倍型。诊断 地中海贫血α 3.7和α 4.2缺失将由Southern BamHI酶切后进行印迹。此外，核心将帮助 该中心的研究人员进行基因组DNA和RNA分离，质粒 构建、转染和分析基因表达， 报道基因测定、引物延伸和FACSGAL分析。此外 核心将作为中心研究者和核心之间的接口 阿尔伯特·爱因斯坦医学院的设施（寡核苷酸 以及DNA测序、转基因小鼠和敲除生产等）

(Adapted from Applicant's Abstract) The overall aims of the core are to perform diagnostic test for sickle cell anemia and thalassemia at the DNA level and to provide molecular biology support to investigators of the center. The core will perform sickle cell anemia, diagnostic, alpha- thalassemia (alphaSEA, alphaMED, alpha20.5), HPFH-1, HPFH-2, and haplotypes determination using the Polymerase Chain Reaction. Diagnostic thalassemia alpha3.7 and alpha4.2 deletions will be performed by Southern blot after digestion by BamHI. In addition, the core will help investigators of the center perform genomic DNA and RNA isolation, plasmid constructions, transfections and analysis of gene expression using reporter assay, primer extension and FACSGAL analysis. In addition the core will serve as an interface between center investigators and the core facilities of the Albert Einstein College of Medicine (Oligonucleotides and DNA sequencing, transgenic mice and knock-out production etc.)