A muSI-RIAC instrument for multi-bioanalyte assays

描述(由申请者提供)：这项资助的目的是开发一种MUSI-RIAC仪器，对微量的样本进行循环免疫亲和层析，如尿液、血浆或其他基于生物的液体。该项目将结合微量免疫亲和层析的优越特性和微量顺序注射的高精度能力，为临床诊断和生物医学分析创建一个非常通用和低成本的仪器平台。 MUSI-RIAC仪器将为临床医生和研究人员提供筛选低丰度目标分析物的复杂基质的能力。该设备在包括生物技术和药物发现、免疫学和诊断性临床分析在内的许多生物医学领域具有巨大的潜力。该设备将使用极少量的样品，并通过多个微亲和色谱柱快速处理，以分离并顺序测量目标分子的存在。对公众健康的好处将影响研究发现的进步以及传统的临床诊断。 需要对儿科、产后或老年患者进行常规多分析物分析的临床情况可能需要使用常规技术进行过量采样。通过Musi-RIAC仪器能够用一滴液体(例如血液、脑、脊髓或淋巴液)进行所有所需的分析，可以极大地减少对患者健康和护理的严重不适或危险。 研究发现常常受制于需要对价值很高但数量很少的复杂样本进行多组分分析。例如，用于药物发现、存档血液样本或细胞培养提取物的重组化学。筛选低丰度目标分子、分离、定量和回收这些组分的能力将极大地促进研究界识别和研究有助于推进各自领域的重要生物分析物相互作用的能力。

DESCRIPTION (provided by applicant): The aim of this grant is the development of a muSI-RIAC instrument that performs recycles immunoaffinity chromatography on a micro scale amount of sample such as urine, plasma or other biological based fluids. This project will synergistically combine the superior attributes of micro immunoaffinity chromatography with the high precision capabilities of micro sequential injection to create a very versatile and low cost instrumental platform for clinical diagnostics and biomedical analysis. The muSI-RIAC instrument will provide clinicians and researchers the ability to screen complex matrices for low abundance target analytes. This device holds great potential in many biomedical endeavors including biotechnology and drug discovery, immunology, and diagnostic clinical analysis. This device will use miniscule amounts of sample and quickly process it through multiple micro-affinity columns to isolate and then sequentially measure for the presence of targeted molecules. Benefits to the public health will impact both advancements in Research discovery as well as conventional Clinical diagnostics. Clinical situations where routine multi-analyte assays are required on pediatric, post-natal, or elderly patients may require excess sampling using conventional techniques. The serious discomfort or danger to the patients health and care can be greatly reduced through the muSI-RIAC instrument's ability to perform all needed assays with a single drop of fluid (e.g. blood, cerebral, spinal or lymphatic fluids). Research discovery is often encumbered by the need to perform multiple component analysis from highly valued yet small amounts of complex samples. For example, recombinant chemistries for drug discovery, archival blood samples, or cellular culture extracts. The ability to screen for low abundant target molecules, isolate, quantitative and retrieve such components would highly benefit the research communities ability to identify and study important bio-analyte interactions that serve to advance their respective fields.