Core--Recombinant technologies

描述：重组技术核心被设想为 试剂、设备和技术专长，用于需要 DNA和其他生物活性分子的细胞转染法。它 应该强调的是，该核心不是作为服务设施而设计的，并且 这些实验将由个人中心的成员进行 实验室在核心人员的指导和监督下。 核心的特点是它的四个目标：1)一个媒介水库，用于 试剂，主要是病毒库存和包装提取物，用于 改造；2)有罩和孵化器支撑的粒子枪；3)a) 小细胞注射设施，包括能够 操纵小鼠细胞、小鼠卵子、果蝇胚胎，并进行培养 以及4)卵母细胞注射设备，设计用于生产和 注射青蛙卵母细胞。为了实现这些目标，核心将 维护一些设备，包括三个层流罩 (目前正在运行)；细胞培养孵化器(3个已到位，1个即将投入使用 由本申请提供)；“基因枪”(已购买)；倒置的 荧光显微镜(根据本申请购买)；以及 微量注射站(显微镜、移液器、机械手、稳定工作台、 全部由本申请提供)。核心将在 由本应用程序支持的一名工作人员科学家进行日常工作；以年为单位 -04和-05，一旦小细胞注射设施运行， 还需要一名半职研究技术员的服务。

DESCRIPTION: The Recombinant Technologies Core is envisioned as a source of reagents, equipment, and technical expertise for projects requiring the cellular transfection of DNA and other biologically active molecules. It should be emphasized that this Core is not designed as a service facility, and that experiments are to be performed by the members of the individual Center laboratories under the guidance and supervision of core personnel. The Core is characterized by its four objectives: 1) a vector reservoir for reagents, primarily viral stocks and packaging extracts to be used in transformations; 2) a particle gun supported with hoods and incubators; 3) a small cell injection facility, including an injection apparatus capable of manipulating mouse cells, mouse eggs, drosophila embryos, and cultured neurons; and 4) an oocyte injection facility, designed for the production and injection of frog oocytes. To realize these objectives, the Core will maintain a number of pieces of equipment, including three laminar flow hoods (currently operational); cell culture incubators (three in place, one to be provided by this application); a "gene gun" (already purchased); an inverted fluorescent microscope (to be purchased under this application); and a microinjection station (microscope, pipette puller, manipulator, stable-table, all to be supplied by this application). The Core will be managed on a day-to-day basis by a staff scientist supported by this application; in Years -04 and -05, once the small cell injection facility is operational, the services of a half-time research technician is also requested.